猪精液冷冻保存程序的优化  

Optimization of Procedure in Boar Semen Cryopreservation

作　　者：胡传活[1,2] 王献伟[1,2] 胡传水[3] 凌泽继[2] 卢晟盛[1,2] 卢克焕[1,2] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530005 [2]广西大学动物科技学院,广西南宁530005 [3]广西农业职业技术学院,广西南宁530007

出　　处：《西南农业学报》2011年第4期1534-1537,共4页Southwest China Journal of Agricultural Sciences

基　　金：广西大学科研基金资助项目(X081027;XBZ091008);广西教育厅科研项目(201012MS001)

摘　　要：研究共4个试验:①按入氮温度分为-5、-30、-60、-90、-120℃5个组;②按鲜精液预处理方式分为未预稀释和预稀释2个组;③按解冻温度设37、50、70℃3个组;④按稀释后精子密度分为1.25×108、2.50×108、5.00×108spz/mL 3个组。结果表明:①不同入氮温度下精子冻后活力差异不显著,但随着入氮温度的降低,精子冻后活力呈升高趋势;②预稀释不能显著提高平衡后精子活力,但可以显著或极显著提高精子稀释后活力、冻后活力、VAP、VSL及VCL等运动指标;③3种解冻温度在精子冻后活力、VAP、VSL、VCL等4个冻后运动指标上没有显著差异,但是,随着解冻温度的上升,冻后活力呈上升趋势;④3种稀释后精子密度在猪精子冻后活力、VAP、VSL上没有显著差异,但随着密度的上升,冻后精子活力及VAP呈下降趋势,而且最高密度组精子的VCL显著慢于其它两组。总之,5种入氮温度中,以-120℃组精子的冻后活力最高;预稀释对于新鲜猪精液的冷冻保存很有必要;3种解冻温度中,70℃组精子冻后活力最高;而高密度不利于猪精子的冷冻保存。Four experiments were included in the present study. Five, two, three and three treatments were designed for experiment 1 （ plunging temperatures： -5, -30, -60, -90 and - 120 ℃ ）, experiment 2 （fresh semen preprocessing： nonpre-extending and pre- extencling） , experiment 3 （ thawing temperatures： 37, 50 ℃ and 70 ℃ ） , and experiment 4 （ extended-spermatozoa concentration： 1.25 × 108, 2.50 ×108 and 5.00 × 108 spz/mL） respectively. The results showed that （ i ） the differences of spermatozoa motility at different plunging temperatures were not significant, but motility of frozen-thawed spermatozoa revealed a tendency to improve with the drop of plunging temperature （ ii ） the preliminary dilution did not significantly increase motility of extended spermatozoa, but it made motility of equilibrated spermatozoa, motility, VAP, VSL and VCL of frezen-thawed spermatozoa significantly or very significantly higher, （iii） there were no significant differences among the three thawing temperatures in motility, VAP, VSL and VCL of frozen-thawed spermatozoa, however, the higher the thawing temperature was, the higher the motility of frozen-thawed spermatozoa was and （iv） three different concentrations of extended spermatozoa did not result in significantly different motility, VAP and VSL of frozen-thawed spermatozoa; ncvertbeless, the motility and VAP fell with the enhancement of extendedspermatozoa concentration, and the highest extended-spermatozoa concentration brought significantly lower VCL of frezen-thawed spermatozoa than the two other treatments. In conclusion, of the five plunging temperatures of the straw, the optimal one was - 120 ℃, where the best sperm motility could be observed after thawing. And the preliminary dilution of fresh semen before extending was indispensable to cryopreservation of boar semen; besides, the highest motility of spermatozoa, thawed at 37. 50 and 70 ℃ respectively, would be detected in the group 70 ℃, and moreover, high diluted-spermato

关 键 词：猪 精液 冷冻保存 程序 运动参数 

分 类 号：S828[农业科学—畜牧学]