凡纳滨对虾肌球蛋白轻链基因MLC的克隆及表达分析  

Clone and Expression Analysis of Myosin Light Chain in Litopenaeus vannamei

作　　者：李文兰[1] 盛小伟[1,2] 高永华[1,2] 陈晓汉 马宁 熊建华 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541000 [2]广西壮族自治区水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁530021

出　　处：《西南农业学报》2011年第4期1558-1563,共6页Southwest China Journal of Agricultural Sciences

基　　金：广西直属公益性科研院所基本科研业务费(2060302GXIF-2008-02);国家虾产业技术体系岗位科学家经费资助(nycytx-46);广西自然科学基金项目(0991106)

摘　　要：以生长性状分离凡纳滨对虾家系的肌肉组织,构建凡纳滨对虾生长性状差减cDNA文库,斑点杂交筛选获得MLC基因,克隆获得全长编码区序列。序列分析表明序列包含462 bp的开放阅读框,推测编码的蛋白质为153氨基酸,预测的分子量为17.5ku,等电点为4.67。通过荧光定量PCR的方法研究了MLC基因在凡纳滨对虾不同组织和不同生长期的肌肉组织中表达情况,显示MLC基因在凡纳滨对虾的心脏、肝胰腺、肌肉、胃、肠、眼柄、鳃和血等组织或器官中都有表达,在肌肉组织中表达量最高,在肝胰腺中表达量最低;在日龄为45 d的对虾肌肉组织中表达量最高,在日龄为10 d的对虾肌肉组织中表达量最低。A subtracted eDNA libraries of Litopenaevs varnamei on growth traits was constructed, and MLC gone of Litopenaeus vannamei was gained by dot blot and cloning. The sequence contains a 462bp open reading frame （ORF） encoding 153 amino acids（as）. The predicted molecular weight is 17.5 ku and the theoretical isoelectric point 4.67. Quantitative real-time PCR was used to assess the mRNA expression of MLC in different tissue and different growth period. Its expression was detected in heart, hcpatopancreas, muscles, stomach, bowel, eye hilt, gill and blood. The highest expression level was observed in muscle, while the lowest in hepatopancreas; the expression of MLC in different growth showed that the highest expression level in muscles was 45 days age, while the lowest was 10 days age.

关 键 词：凡纳滨对虾 肌球蛋白轻链 抑制性差减杂交 荧光定量PCR 

分 类 号：S945.46[农业科学—水产养殖]