镜鲤体重的QTL定位  

Mapping QTLs Related to Body Weight of Mirror Carp (Cyprinus carpio L.)

作　　者：李文升[1,2] 刘翠[1,3] 张晓峰[1] 张天奇[1,4] 王宣朋[1,2] 储志远[1,4] 孙效文[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306 [3]哈尔滨师范大学,哈尔滨150025 [4]大连海洋大学生命科学与技术学院,大连116023

出　　处：《基因组学与应用生物学》2011年第4期316-324,共9页Genomics and Applied Biology

基　　金：国家重大基础研究发展规划(973)项目"重要养殖鱼类功能基因组和分子设计育种的基础研究"(No.2010CB126305);黑龙江水产研究所基本科研业务费专项资金(No.2009HSYZX-SJ-08)

摘　　要：本研究利用217个微卫星标记和336个SNPs标记对德国镜鲤F2代68个个体基因组DNA进行基因型检测。其中507个标记共组成62个连锁群,覆盖基因组总长度为2805.85cM,标记间平均距离为6.31cM;利用软件MapQTL4.0采用区间作图法对体重性状进行QTL定位分析。研究结果共检测到14个与体重性状有关的QTLs,分布于9个连锁群。其中BW-5-1有最大的LOD值,为4.46;BW-1-1的LOD值最小,为2.25。单个QTL平均解释表型变异介于14.10%～45.50%之间,其中贡献率大于20%的主效QTLs有9个。通过BLASTX与斑马鱼蛋白质序列数据库进行序列比对,找到了与斑马鱼酰基辅酶A脱氢酶蛋白、胰淀粉酶α2蛋白、Apoeb protein和甘油醛-3-磷酸脱氢酶蛋白同源的分子标记。本研究结果对分子标记辅助育种具有重要应用价值。In this study, 217 microsatellite markers and 336 SNPs markers were selected to analyze the genomic DNA of 68 individuals derived from the group of F2 hybrids mirror carp （Cyprinus carpio L.）. A genetic linkage map was constructed using 507 markers. The map spaned 2 805.85 cM over 62 linkage groups, with an average spacing of 17.07 cM. Then quantitative trait loci （QTLs） associated with body weight were identified by the interval mapping of the software MapQTL 4.0. Fourteen QTLs were identified for body weight on nine linkage groups. Among the existence of fourteen QTLs controlling body weight in mirror carp the maximum LOD score was found to be 4.46 （BW-5-1） and the minimum score was found to be 2.25 （BIV-I-1）. The variances explained by these QTLs ranged from 14.10% to 45.50% and nine major QTLs explained over 20% of the phenotypic variations. By BLASTX with the zebrafish protein sequence database for sequence alignment, and found with the zebra fish protein acyl-coenzyme a dehydrogenase, amylase α2 protein, apoeb protein and glyceraldehyde-3-phosphate dehydrogenase protein homologous molecular markers. The results could be well used for molecular marker as- sisted selection and breeding.

关 键 词：镜鲤 体重 数量性状基因座 分子标记辅助 

分 类 号：S917.4[农业科学—水产科学] Q75[生物学—分子生物学]