阿托伐他汀对急性百草枯中毒鼠肺p38MAPK表达及肺损伤的影响  

Protective effect of atorvastatin on paraquat-induced injury in rat pulmonary microvascular endothelial cells expression of p38MAPK in rats

作　　者：刘明伟[1] 苏美仙[2] 李岚[1] 何杰明[1] 

机构地区：[1]云南省昆明市延安医院呼吸二科,650051 [2]昆明医学院第二附属医院SICU,650101

出　　处：《重庆医学》2011年第25期2534-2536,I0001,共4页Chongqing medicine

摘　　要：目的探讨阿托伐他汀对大鼠百草枯(PQ)中毒急性肺损伤(ALI)的保护作用及其机制。方法选择健康SD大鼠60只,随机分为3组:PQ组、阿托伐他汀组和对照组。PQ组和阿托伐他汀组腹腔内注射PQ,阿托伐他汀组给予阿托伐他汀1.5mg/(kg.d)灌胃治疗;对照组注射等体积无菌生理盐水。7 d后测支气管肺组织p38丝裂原活化蛋白激酶(p38MAPK)的表达、核因子-κB(NF-κB)活性细胞、支气管肺泡灌洗液(BALF)中炎症细胞及血清中MMP-9、TNF-α和IL-6水平。结果 PQ组和阿托伐他汀组支气管肺组织p38MAPK的表达,NF-κB活性细胞,支气管BALF中总细胞数、中性粒细胞数、巨噬细胞数均较对照组增高(P<0.05),阿托伐他汀组各指标明显低于PQ组(P<0.05)。结论阿托伐他汀可抑制p38MAPK的表达,进一步抑制NF-κB及炎症细胞活性,对PQ中毒大鼠ALI有治疗作用。Objective To study the protective effect of atorvastatin on paraquat-induced injury in rats and the mechanism.Methods Sixty rats were enrolled and randomly divided into three groups：contrast group,paraquat group and atorvastatin group.Peritoneal paraquat injection were performed in paraquat group and atorvastatin group,while equal volume of saline was peritonealy injected in contrast group,atorvastatin group injected with paraquat 12 h,given atorvastatin 1.5 mg/（kg·d）by the injection tube.Seven days later,the expression of p38MAPK,the nurmber of NF-κB positive cells,inflammatory cells in bronchoalveolar lavage fluid（BALF）and the levels of IL-6 and TNF-α in the serum were measured.Results The expression of p38MAPK,the nurmber of NF-κB positive cells,the total number of cells,neutrophilic granulocytes,macrophage in BALF and the levels of IL-6 and TNF-α in the serum were significantly increased in paraquat group and atorvastatin group（P0.05）.But their levels in atorvastatin group were lower than that in paraquat group（P0.05）.Conclusion In paraquat-induced acute lung injury rat models,atorvastatin can alleviate inflammatory response and lung injury,inhibit the expression of p38MAPK and activity of NF-κB,possess potential protective effects.

关 键 词：阿托伐他汀 肺损伤 百草枯 P38MAPK 

分 类 号：R965[医药卫生—药理学]