乌司他丁对严重烧伤大鼠肠道损伤及炎症因子的影响  

Effects of ulinastatin on intestine damage and inflammatory factors in severely burned rats

作　　者：孙勇[1] 孙曙光[1] 王静[1] 王良喜[1] 毛学飞[1] 邓向东[1] 潘晓峰[1] 吴杭庆[1] 

机构地区：[1]中国人民解放军第97医院烧伤整形科,江苏徐州221004

出　　处：《重庆医学》2011年第26期2601-2602,2605,共3页Chongqing medicine

基　　金：2007年度南京军区医学科研课题资助项目(07m028)

摘　　要：目的观察乌司他丁对严重烧伤大鼠致肠黏膜损害和炎症因子的影响。方法采用30%体表面积Ⅲ度烧伤大鼠模型,随机分成烧伤对照组、乌司他丁组,两组给予等氮、等热量、等体积的营养液。乌司他丁组于伤后每日静脉注射乌司他丁。观察烧伤前及烧伤后第1、2、3、4、5、6、7天大鼠的氮平衡变化,同时检测烧伤后血浆D-乳酸、二胺氧化酶(DAO)活性及肿瘤坏死因子α(TNF-α)、白介素6(IL-6)的含量。结果烧伤后两组大鼠的氮平衡、D-乳酸、DAO、TNF-α及IL-6均显著高于烧伤前(P<0.01);乌司他丁组的氮平衡、D-乳酸及DAO活性与烧伤对照组相似(P>0.05);而TNF-α及IL-6含量显著低于烧伤对照组(P<0.05,P<0.01)。结论乌司他丁能有效降低血浆炎症因子水平,而对肠黏膜屏障没有直接的保护作用。Objective To explore the effect of ulinastatin on intestine damage and inflammatory factors after severe burn injury. Methods 90 wistar rats were inflicted with 30% total body surface area full thickness burns and then randomly divided into burned control group and ulinastatin treatment group. Isonitrogenous and isocaloric nutrition supports were provided to both groups. Rats in ulinastatin treatment group were treated with ulinastation 40 000 IU/kg via tail vein injection. And then we investigated the changes of the nitrogen balance before rats burned and seven days after burned. Nitrogen balance, D-lactate, diamine Oxidase（DAO）, and the levels of plasma TNF,IL-6 were determined. Results After burn injury,the nitrogen balance,D-lactate,DAO activity, and levels of TNF,IL-6 significantly increased. Nitrogen balance,D-lactate and DAO activity in U group were similar to those in B group, while levers of TNF and IL 6 were significantly lower compared with B group. Conclusion Ulinastatin may lower the level of plasma inflammatory cytokines,while can not relieve intestine damage after severe burn injury.

关 键 词：烧伤 肠黏膜 乌司他丁 炎症因子 

分 类 号：R965[医药卫生—药理学]