超声联合反义c-myc寡核苷酸对移植静脉内膜增生的影响  

Effect of ultrasound conjunction with antisense c-myc oligodeoxyuncleotides on vein graft intimal hyperplasia

作　　者：刘杨东[1] 赵渝[1] 时德[1] 彭真年[2] 廖晓刚[3] 

机构地区：[1]重庆医科大学附属第一医院血管外科,400016 [2]重庆医科大学病理教研室,400016 [3]重庆医科大学电镜教研室,400016

出　　处：《重庆医学》2011年第26期2603-2605,2596,共3页Chongqing medicine

摘　　要：目的观察超声辐照联合局部应用反义c-myc寡核苷酸对移植静脉内膜增生的影响,以期寻找防治内膜增生的新方法。方法将24只兔随机分为4组,对照组、超声辐照组、反义核苷酸组、超声辐照加反义核苷酸组,每组6只,建立兔自体颈内静脉移植替代颈总动脉模型。超声辐照组术后当天至第7天使用超声(2.190 W/cm2,0.8 MHz)辐照移植血管段,反义核苷酸组局部转染反义c-myc寡核苷酸。观察移植静脉的组织形态学改变;增殖细胞核抗原(PCNA);反转录PCR(RT-PCR)检测移植血管段c-myc基因的表达。结果术后第4周超声辐照组、反义核苷酸组、超声辐照加反义核苷酸组内膜厚度、PCNA阳性细胞数、c-myc基因表达均明显低于对照组(P<0.01)。结论超声辐照和局部转染反义c-myc可以降低平滑肌细胞的增殖和减轻内膜增生,可作为静脉移植后再狭窄的防治方法。Objective To assess the effect of ultrasound conjunction with antisense c-myc oligodeoxyuncleotides treatment on intimal hyperplasia in a vein autograft model which is to find the new method in prevention of intimal hyperplasia. Methods 24 ra bbits were divided into 4 groups randomly,which including of control group, ultrasound group, antisense c-myc group and ultrasound conjunction with antisense c-myc oligodeaxynucleotides group,and 6 rabbits in each group. Fabricating a common carotid ar tery interposition model with ipsilateral internal jugular vein. The vein grafts treated by ultrasound （2. 190 w/cm^2,0.8 MHz） and ultrasound conjunction with antisence c-myc oligodeoxynucleotides. Neointimal thickness was calculated by histomorphometric quantification,expression of PCNA by immunostainning and c myc expression by RT-PCR. Results Compared with the control group,after the fourth week the neointimal thickness,the number of PCNA positive ceils and c myc expression in ultrasound,antisense and ultrasound ＋antisense other groups were decreased significantly （P〈0.01）. Conclusion Treatment with ultrasound and antisense c myc oligodeoxynucleotides seemed to decrease intimal hyperplasia and decelerate SMC proliferation. Therefore, ultra sound conjunction With antisense c-myc oligodeoxyuncleotides might be an effective treatment to reduce vein graft restenosis.

关 键 词：增生 肌 平滑 血管 兔 超声检查 静脉移植 自体 反义c—myc寡核苷酸 

分 类 号：R654.3[医药卫生—外科学]