超敏C反应蛋白、T淋巴细胞亚群在妊娠糖尿病诊治中的应用价值  

Value of Hs-CRP,T Lymphocyte Subsets in the Gestational Diabetes Diagnosis and Treatment

作　　者：陈大宇[1] 潘柳吟[1] 

机构地区：[1]广西柳州市人民医院检验科,545001

出　　处：《重庆医学》2011年第26期2618-2619,共2页Chongqing medicine

摘　　要：目的探讨妊娠糖尿病患者外周血的超敏C反应蛋白(hs-CRP)、T淋巴细胞亚群在妊娠糖尿病诊治过程中的应用价值。方法选择2009年10月至2010年4月在本院产科门诊妊娠24～28周的孕妇,进行50 g葡萄糖负荷试验(GCT)孕妇72例为GDM组,葡萄糖耐量试验(OGTT)正常孕妇68例为对照组,hs-CRP采用乳胶增强免疫比浊定量法测量,葡萄糖采用己糖激酶法测定;应用CD3、CD4、CD8单克隆抗体进行间接免疫荧光检测。检测hs-CRP和T淋巴细胞亚群,并分析hs-CRP和T淋巴细胞亚群的相关性。结果 GDM组hs-CRP为(5.14±0.75)mg/L比NGT组高(P<0.01);两组CD3、CD4、CD8和CD4/CD8比较,差异有统计学意义(P<0.01);GDM患者hs-CRP与CD3、CD4呈负相关(r=-0.652)。结论 hs-CRP与T淋巴细胞亚群在GDM的辅助诊断及病情判断方面有重要的临床价值。Objective To study the value of the high sensitivity CRP,T lymphocyte subsets in gestational diabetes diagnosis and treatment. Methods 150 cases of 24-28 week＇s pregnant women chosen from october,2009 to April,2010 in the obstetrics depart ment of our hospital were divided into two groups. With abnormal glucose challenge lest （GCT）of 50 g, 72 cases of gestational dia betes were chosen as GDM group,and 68 normal pregnant women as NGT group. Hs-CRP was measured by latex enhanced immunoturbidimetric quantification; and the glucose was measured by hexokinase, indirect immunofluorescence assay was applied in CD3,CD4,CD8 monoelonal Antibodies to detect hs-CRP and T lymphocyte subsets and analyze their correlation. Results H s CRP of GDM group was 5.14±0.75 mg/L which is higher than that of NGT group （P〈0.01）;there is statistical difference between the CD3,CD4,CD8 and CD4/CD8 of the two groups （P〈0.01）;and there is a negative correlation relationship between hs CRP and CD3,CD4 of GDM group （r=-0. 652）. Conclusion Hs-CRP and T lymphocyte subsets in the diagnosis and progression of GDM judgments have important clinical value.

关 键 词：糖尿病 妊娠 C反应蛋白质 T淋巴细胞 

分 类 号：R714.256[医药卫生—妇产科学]