三种品系白纹伊蚊转铁蛋白基因表达量的比较分析  

Expression level comparative analysis of the transferrin gene in 3 strains of Aedes Aegypti

作　　者：王霄[1] 谭文彬[2] 程鹏[3] 公茂庆[3] 刘丽娟[3] 王海防[3] 全芯[1] 高红刚[1] 

机构地区：[1]济宁医学院:临床技能中心,山东济宁272013 [2]济宁医学院:病原生物学教研室,山东济宁272013 [3]山东省寄生虫病防治研究所,山东济宁272013

出　　处：《国外医学（医学地理分册）》2011年第3期182-183,187,共3页Foreign Medical Sciences：Section of Medgeography

基　　金：山东省教育厅课题资助项目(No.JO9LF04);济宁市科委课题资助项目(No.2009;2010)

摘　　要：目的进行不同品系白纹伊蚊转铁蛋白基因表达量的分析。方法据二维电泳获得肽段序列设计引物扩增基因片段,荧光定量PCR检测在白纹伊蚊抗性品系、敏感品系、现场采集品系蚊体内的表达水平差异。结果成功扩增白纹伊蚊转铁蛋白基因片段,荧光定量PCR表明转铁蛋白基因在抗性品系蚊体内的表达量是敏感品系的10.51倍,现场品系是敏感品系的5.22倍。结论白纹伊蚊转铁蛋白基因可做为新的抗性检测及治理基因靶标。Objective Analysis the transferrin gene expression level in different stains of Aedes Aegypti. Methods Degenerate primers were designed on the basis of two-dimensional gel electrophoresis peptide fragment sequence, fluorescence quantitative PCR detect the expression level difference between resistant strain, sensitive strain and local strain of Aedes Aegypti. Results The transferrin gene of Aedes Aegypti was cloned successfully. FQ-PCR proved the transferrin gene expression level of resistant strain is 10.51 times higher than that of sensitive strain, while the expression level of local strain is 5.22 times higher than that of sensitive strain. Conclusion Aedes Aegypti transferrin gene can be used as a new gene target for resistance detection and treatment.

关 键 词：白纹伊蚊 转铁蛋白 荧光定量PCR 

分 类 号：R183.5[医药卫生—流行病学] Q969.44[医药卫生—公共卫生与预防医学]