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作 者:赖杭桂[1] 朱楠楠[1] 陈庚华[1] 韩平原[1] 蔡秀清[1]
出 处:《热带农业科学》2011年第7期29-33,38,共6页Chinese Journal of Tropical Agriculture
基 金:中央级公益性科研院所基本科研业务费专项(NoPZS062-03);"211工程"热带作物遗传育种与生态保育创新人才培养基金(No.QNJS-2011-05)
摘 要:以木薯华南5号、华南7号、华南8号、面包木薯以及多倍体8229为材料,分析了这5个木薯品种的基本农艺性状、叶片叶绿素含量和品种抗性,同时利用RAPD以及过氧化物同工酶分析了这5个木薯品种的亲缘关系。结果表明:华南7号与面包木薯生长优势明显,株高、茎粗显著高于其它3个品种。多倍体8229小裂叶呈短粗状、叶柄较短、叶形指数较小,低温处理后相对电解质渗出率最低,表明该品种对低温的抗性相对较强。叶片叶绿素含量以华南8号最高,达到4.63 mg/g,显著高于其余4个品种;RAPD分析技术和过氧化物同工酶分析结果表明:引物S76能够有效的对5个木薯品种进行分离和鉴定,华南5号与华南7号的亲缘关系较近,在分子水平存在较大的相似性。Five cassava varieties of South China 5(SC5),SC7,SC8,Bread cassava and Polyploid 8229 were used in this research.The agronomic traits,chlorophyll content and evaluation of cold resistance have been done,and the genetic relationship has been analyzed by using RAPD as well as peroxidase isozyme analysis technique.The results showed that the varieties of Bread cassava and SC7 had significantly better agronomic traits than the other three ones such as higher in plant height and bigger in stem diameter.The dehiscent leaves of the Polyploid 8229 are shorter in length,but wider in width;with shorter stem and lesser dehiscent number;Meanwhile it had smaller electrolytic exudation rate after cold stress,which suggests that the Polyploid 8229 is better in cold resistance.The chlorophyll content was the highest in SC8(4.63 mg/g),compare to other species.The results showed that the primer S76 could functionally identify the five Varieties of Cassava by RAPD.SC5 and SC7 show close genetic relationship from the RAPD and peroxidase isozyme analyses.
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