不同NSAIDs肠病模型中肠黏膜通透性的比较研究  

The change of intestinal permeability in different NSAIDs enteropathy models

作　　者：刁磊[1] 梅俏[1] 许建明[1] 

机构地区：[1]安徽医科大学第一附属医院消化内科,安徽合肥230022

出　　处：《安徽医药》2011年第10期1209-1211,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨不同NSAIDs肠病模型中肠黏膜通透性改变的研究。方法选用不同NSAIDs(阿司匹林、吲哚美辛、双氯芬酸钠)制备小鼠NSAIDs肠病模型。其中阿司匹林组(500 mg.kg-1)、吲哚美辛组(5 mg.kg-1)、双氯芬酸钠(10 mg.kg-1)灌胃给药3 d。在此基础上,进行不同剂量双氯芬酸钠不同时间灌胃给药。实验结束后取小鼠小肠进行Evans-blue通透性检测,并计算小肠溃疡数目。结果在不同NSAIDs肠病模型中,阿司匹林组小鼠小肠黏膜未见明显溃疡,小肠黏膜通透性轻度升高(621.1±114.2 vs 460.6±89.7μg.g-1),吲哚美辛组小鼠小肠黏膜损害明显,小肠溃疡明显增多(8.2±1.9 vs 0),小肠通透性显著升高(2132.0±315.7 vs 460.6±89.7μg.g-1),双氯芬酸钠组小肠黏膜损伤和小肠黏膜通透性升高均较吲哚美辛组减轻。随着双氯芬酸钠给药剂量增加和给药时间延长,小鼠小肠黏膜损害和肠黏膜通透性增高越明显。结论在不同NSAIDs肠病模型中,阿司匹林组小鼠小肠损害轻,吲哚美辛组小肠损伤严重,双氯芬酸钠2.5 mg.kg-1灌胃3 d可引起小肠黏膜通透性和肠黏膜损害,适用于NSAIDs肠病模型的研究。Aim To investigate the change of intestinal permeability in different NSAIDs enteropathy models.Methods NSAIDs enteropathy models were induced by different NSAIDs（aspirin,indomethacin,diclofenac）.Aspirin group（500 mg·kg-1）,indomethacin group（5 mg·kg-1）,diclofenac group（10 mg·kg-1） were administered by gavage for 3 days.Diclofenac was administered orally at different times and doses.Evans-blue permeability test was detected in the small intestine of mice at the end of experiment,and the number of ulcers was calculated.Results In the different NSAIDs enteropathy models,the aspirin group did not have any ulceration,the small intestinal permeability slightly elevated（621.1 ± 114.2 vs 460.6 ± 89.7 μg·g-1）.Indomethacin significantly induced the small intestinal damage in mice,which showed an significantly increased ulcers（8.2 ± 1.9 vs 0） and higher intestinal permeability（2 132.0 ± 315.7 vs 460.6 ± 89.7 μg·g-1）.Compared with indomethacin group,the small intestinal mucosal injury and intestinal mucosa permeability were reduced in diclofenac group.The small intestinal damage and permeability turned to be more apparent with the doses and times increased.Conclusion In different NSAIDs enteropathy models,aspirin induced a slight damage,while the severe intestinal damage were seen in indomethacin group.Diclofenac 2.5 mg·kg-1 orally three days can cause increased small intestinal permeability and intestinal damage,which is suitable for the study of NSAIDs enteropathy model.

关 键 词：阿司匹林 吲哚美辛 双氯芬酸钠 肠黏膜通透性 

分 类 号：R965[医药卫生—药理学]