苦瓜植株不同组织中降糖血糖多肽活性成分的RT-PCR分析  

RT-PCR Analysis of Hypoglycemic Polypeptide Components from Different Tissues of Momordica charantia

作　　者：宋还雷[1] 谢先文 顾红艳 任大云[1] 王富军[3] 赵健[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]浙江日升昌药业有限公司,浙江东阳322100 [3]上海中医药大学,上海201203

出　　处：《食品与药品》2011年第9期312-315,共4页Food and Drug

基　　金：国家科技支撑计划子课题(No.2006BAI06A16-01)

摘　　要：目的研究苦瓜降糖活性多肽P在苦瓜不同组织器官中的分布。方法用改良Trizol一步法提取苦瓜不同组织的总RNA,采用RT-PCR方法扩增降糖多肽P基因,分析其表达。结果成熟果实的种子和果肉中均可明显检测到降糖多肽P基因的表达,其他组织中则未能检测出。结论苦瓜降糖多肽P活性成分主要在苦瓜果肉和种子中表达,这2种组织是苦瓜降血糖作用的主要药用部位。Objective To investigate the distribution of hypoglycemic polypeptide P in different tissues of Momordica charantia （MC）. Methods The improved one-step Trizol extraction method was used for the isolation of total RNA from different tissues of MC. A RT-PCR method was used for analyzing the expression patterns of the hypoglycemie polypeptide P gene in MC. Results The RT-PCR analysis results showed that the expression of hypoglycemie polypeptide P gene could only be detected in seeds and fruit pulp of mature MC. Conclusion The hypoglycemic polypeptide P mainly expresses in seeds and fruit pulp of MC. These two tissues are the main medicinal parts for hypoglycemic therapy of MC.

关 键 词：苦瓜 RNA提取 RT-PCR 降糖多肽P表达 

分 类 号：S642.5[农业科学—蔬菜学]