葛根素对糖尿病大鼠胰腺线粒体氧化应激及ATP酶的影响  

Effect of Puerarin on Pancreatic Mitochondria Oxidative Stress and ATPase in Diabetic Rats

作　　者：孙卫[1] 郑学芝[1] 李丽[1] 刘桂莲[1] 念红[1] 徐秋玲[1] 

机构地区：[1]牡丹江医学院生理教研室,黑龙江省牡丹江市157011

出　　处：《临床合理用药杂志》2011年第08X期13-14,共2页Chinese Journal of Clinical Rational Drug Use

基　　金：黑龙江省卫生厅科研项目(2006-392)

摘　　要：目的探讨葛根素对糖尿病大鼠胰腺线粒体氧化应激及ATP酶的影响。方法将30只Wistar大鼠分为正常对照组、糖尿病组(DM组)和糖尿病治疗组(Pue组,采用葛根素注射液治疗),前两组注射等体积的0.9%氯化钠溶液。8周后,测3组大鼠血清葡萄糖、胰岛素、胰腺线粒体丙二醛(MDA)水平及超氧化物歧化酶(SOD)、Na+-K+-ATP酶(Na+-K+-ATPase)和Ca2+-ATP酶(Ca2+-ATPase)的活性。结果 (1)DM组大鼠血糖和MDA含量明显高于对照组(P<0.001),而血清胰岛素水平、SOD、Na+-K+-ATPase和Ca2+-ATPase活性显著降低(P<0.05)。(2)Pue组大鼠血糖及MDA含量较DM组显著降低(P<0.05),血清胰岛素水平、SOD、Na+-K+-ATPase和Ca2+-ATPase活性显著升高(P<0.05),差别有统计学意义。结论葛根素可减轻糖尿病大鼠胰腺线粒体氧化应激水平。Objective To investigate the effects of puerarin on pancreatic mitochondria oxidative stress and ATPase in diabetic rats. Methods 30 rats were divided into control group, diabetes group and treatment group at random. Diabetic model was reproduced by intraperitoneal injection of alloxan. After having been treated with puerarin for 8 weeks, the serum contents of glucose, insulin, malondialdehyde （MDA） were measured; the activity of superoxide dismutase （SOD）, Na＋- K＋- ATPase and Ca2＋ - ATPase in pancreatic mitochondria were detected respectively. Re.gulls （ 1 ） The contents of glucose, MDA were obviously increased （P 〈0. 001） and the contents of insulin, the activities of SOD, Na＊ -K＋ -ATPase and Ca2＋ -ATPase were obviously decreased （P 〈 0. 001 ） in the diabetes group compared with control group. （2） After therapy with puerarin, the contents of glucose, MDA were obviously decreased（P 〈 0. 0） and the contents of insulin, the activities of SOD, Na ＋ -K ＋ - ATPase and Ca2＋ - ATPase were obviously increased （ P 〈 0. 05 ） in the treatment group compared with diabetes group. Conclusion Puerarin could lighten the pancreatic mitochondria oxidative stress in diabetic rats.

关 键 词：葛根素 糖尿病 线粒体质子转运ATP酶 氧化性应激 

分 类 号：R285.5[医药卫生—中药学]