新疆牛环形泰勒虫虫病的流行病学调查  

Epidemiologic Survey for Detection of Theileria annulata-infected Cattle in Xinjiang by an Improved Indirect Tams1 ELISA Assay

作　　者：简子健[1] 马素贞[1] 孙其喆[1] 沈炯玉[1] 吕伟[1] 苗中秋[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2011年第8期1509-1513,共5页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(3066141)

摘　　要：【目的】2006～2008年全疆14个地州的牛环形泰勒虫病流行病学调查。【方法】使用已建立的牛环形泰勒虫病间接ELISA检测方法,以纯化的重组蛋白GST-Tams1作为抗原。【结果】(1)牛环形泰勒虫病仍是新疆牛梨形病的主要病原。在2006年采集的278份牛血清样品中,阳性血清31份,感染率为11.15%。2007年,在532份牛血清样品中检出阳性血清61份,感染率为11.47%。在2008年的530份牛血清中检出阳性血清61份,感染率为11.51%。(2)2008年,在地方流行性疫病区牛环形泰勒虫感染率高达24%。【结论】新疆牛环形泰勒虫病的特点是疫区分布广,感染率居高不下,保持在11%以上,对动物和人类构成一定的威胁。这是新疆首次利用血清学方法对全疆牛环形泰勒虫病进行大规模的流行病学调查。[Objective and Method]The purpose of this project was to investigate the epidemiology of Theileria annulata - infected cattle in Xinjiang by the improved indirect Tams1 enzyme - linked immunosorbent assay（indirect Tams1 ELISA）.[Result]The results showed that（1） the theileriosis caused by Theileria annulata was still the main causal pathogen in Xinjiang.The infection rates in 2006,2007 and 2008 were 11.15%（31/278）,11.47%（61/532） and 11.51%（61/530） respectively;（2） the infection rate was as high as 24%（15/50） in some regions where the disease occurred in 2008.[Conclusion]The features of bovine theileriosis caused by Theileria annulata in Xinjiang were that profile of epidemic region was largely widespread and the infection rate was kept at high level（〉11%）.It was dangerous for animals and human health.This was a first large - scale epidemiological survey of detecting the specific antibodies against Theileria annulata in cattle in Xinjiang by an improved indirect Tams1 ELISA.

关 键 词：牛 环形泰勒虫 Tams1间接酶联免疫吸附试验 流行病学调查 感染率 

分 类 号：S815.313[农业科学—畜牧学]