铝对大鼠学习记忆及海马神经元AchE活性的影响  

Effect of Aluminum on Learning,Memory and Hippocampal Neurons AchE Activity in Rats

作　　者：许晓利[1] 杨敏[1] 夏保芦[1] 

机构地区：[1]江汉大学医学院基础部,湖北武汉430056

出　　处：《临床医学工程》2011年第9期1341-1342,共2页Clinical Medicine & Engineering

摘　　要：目的观察铝对大鼠空间学习记忆的影响及海马神经元AchE活性的变化,为探讨铝的神经毒性提供实验依据。方法采用Morris水迷宫方法测试染铝大鼠空间学习记忆能力的改变;采用乙酰胆碱酯酶(AchE)组织化学方法对大鼠海马不同亚区AchE神经元活性进行观察。结果大鼠经多次训练后,无论是盐水对照组还是染铝组,其逃避潜伏期均呈缩短趋势。但是大鼠在染铝后30、35、40、45、50、55、60d逃避潜伏期明显大于盐水对照组,两者比较有显著性差异(P<0.01)。染铝后60d大鼠海马不同亚区AchE活性明显降低。结论经多次训练后,大鼠空间学习记忆能力随训练次数增加而有所增强。结果提示铝可影响大鼠空间学习记忆能力,使学习记忆功能降低,其机制可能与海马乙酰胆碱神经元活性的改变有关。Objective To observe the effect of aluminum on spatial learning and memory and the changes of hippocampal neurons AchE activity in rats to provide the experimental evidence for exploring the neurotoxicity of aluminum.Methods The Morris water maze task was used to test the changes of the spacial learning and memorizing ability in the aluminum-exposed rats,the hippocampal neurons AchE activity of rats in different subregions was observed by the use of acetylcholinesterase（AchE） histochemistry.Results After the rats were trained several times,in the saline control group and aluminum-exposed group as well,the escape latency became shorter.But the escape latency in aluminum-exposed rats at 30,35,40,45,50,55,60 days was significantly greater than saline control group（P 0.01）.AchE activity in different hippocampal subregions significantly decreased in 60 days after the rats exposed to aluminum.Conclusion The spatial learning and memory in rats are improved with the increased training times.The results suggest that aluminum can affect the ability of spatial learning and memory in rats,the mechanism of affections of aluminum on neuronal acetylcholine in hippocampus may be related to the change of neuronal activity.

关 键 词：铝 学习记忆 乙酰胆碱酯酶 酶组织化学 

分 类 号：R595.2[医药卫生—内科学]