还少丹对D-半乳糖衰老模型小鼠心肌线粒体DNA缺失的影响  

Effect of Huanshaodan on deletion of myocardial mitochondrial DNA induced by D-gal in aged mice

作　　者：程莉娟[1] 刘群良[1] 谭峰[1] 胡梅[1] 周赛男[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2011年第5期20-22,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅资助项目(07C479)

摘　　要：目的探讨还少丹对D-半乳糖(D-gal)诱导的衰老小鼠心肌线粒体DNA(mtDNA)缺失的影响。方法将40只小鼠随机分为空白对照组、衰老模型组、还少丹低、高剂量组,用D-半乳糖建立衰老模型,分别给予蒸馏水和还少丹水煎液灌胃。连续给药6周后,采用聚合酶链反应(PCR)技术扩增心肌组织mtDNA,经琼脂糖凝胶电泳检测扩增条带,用光密度扫描定量各组mtDNA的缺失率。结果空白对照组未见mtDNA缺失型扩增条带,其余各组均出现不同程度的缺失型扩增条带;与模型组比较,低、高剂量组小鼠心肌mtDNA缺失率明显降低,差异均具有统计学意义(P<0.05);而低剂量与高剂量组之间比较mtDNA的缺失率差异无统计学意义(P>0.05)。结论还少丹能减少D-半乳糖诱导的衰老小鼠心肌mtDNA的缺失程度,提示该方能降低mtDNA的氧化损伤,保护mtDNA的结构完整性,从分子水平上为还少丹抗衰老的作用机制提供了一定的实验依据。Objective To investigate the effect of Huanshaodan on deletion of myocardial mitochondrial DNA induced by D-gal in aged mice. Methods Forty mice were divided randomly into blank control group, aged model group, low dose group and high dose group. The models were established by injecting D-gal and feed with distilled water and Huanshaodan decoction respectively 6 weeks. The polymerase chain reaction（PCR） was used to amplified the myocardial mitochondrial DNA. The agarose gel electrophoresis and light density scanning were used respectively to detect the products of PCR and ratio of mtDNA deletion. Reslilts The mtDNA deletion products were not in bland control group but in all other groups in some degrees. Compared with model group, the mtDNA deletion in both dose groups were significant （P 〈0.05） but difference between the low dose group and high dose group not obvious （P 〉0.05）. Conclusions Huanshaodan can reduce the mtDNA deletion in aged mice, which suggests that the drug can reduce the oxidative damage of mtDNA and protect mtDNA perfection,provides a experimental evidence for the study of anti-aging mechanism of Chinese medicine in molecular level.

关 键 词：还少丹 衰老 心肌 线粒体DNA 小鼠 何首乌 熟地 肉苁蓉 

分 类 号：R285.5[医药卫生—中药学]