内皮中性粒细胞激活肽-78在哮喘大鼠中性粒细胞中的表达及意义  

Expression of epithelial neutrophil activating protein-78 in neutrophil cell of asthma rats and its significance

作　　者：周兴辉[1] 叶辉[2] 罗冬娇[3] 童夏生[4] 阮正英 

机构地区：[1]台州市中西医结合医院检验科,浙江台州317523 [2]台州市第一人民医院儿科,浙江台州317000 [3]杭州师范大学钱江学院医学系,浙江杭州310012 [4]台州市中西医结合医院儿科 [5]病理科,浙江台州317523

出　　处：《中华医院感染学杂志》2011年第18期3791-3794,共4页Chinese Journal of Nosocomiology

基　　金：浙江省卫生厅基金资助项目(2007-B238);温岭市科技局基金资助项目(2006-53)

摘　　要：目的观察大鼠哮喘内皮中性粒细胞激活肽-78(ENA-78),表达及布地奈德对其的影响,探讨中性粒细胞(NEU)参与哮喘发作的可能作用机制。方法采用大鼠哮喘模型,随机分成哮喘组、对照组、布地奈德组,流式细胞术测定血NEU ENA-78的表达。结果哮喘组(97.65±13.99)MFI NEU ENA-78的平均荧光强度显著高于对照组(50.79±8.66)MFI(P<0.01);布地奈德组(75.81±6.10)MFI NEU ENA-78的平均荧光强度显著性低于哮喘组,且高于对照组(均P<0.01),NEU ENA-78的表达水平与支气管肺泡灌洗液(BALF)细胞总数呈显著正相关(n=29,r=0.781,P<0.01)。结论哮喘大鼠NEU ENA-78的表达水平增加,NEU可能通过ENA-78参与哮喘发作的炎症过程;布地奈德可能部分通过下调NEU ENA-78,从而减轻哮喘气道炎症。OBJECTIVE To observe the expression of neutrophil activating protein-78（ENA-78） in asthma rats and the regulation by budesonide.And to investigate the role of neutrophil in the pathogenesis of asthma exacerbation.METHODS In this experiment,the rat model of asthma were randomly divided into three groups on average,including asthma group,control group and budesonide group.Levels of ENA-78 were detected by flow cytometry methods.RESULTS Levels of ENA-78 at blood neutrophil in asthma group（97.65±13.99 mean fluorescence intensity） were significantly higher than those in control group（50.79±8.66 mean fluorescence intensity）（P〈0.01）.And leveles of ENA-78 at blood neutrophil in budesonide group（75.81±6.10 mean fluorescence intensity） were significantly lower than those in asthma group,but higher than those in control group（all P〈0.01）.There were significant positive correlation between ENA-78 expressions at blood neutrophil and numbers of total inflammatory cells in bronchoalveolar lavage fluid（BALF）（n=29,r=0.781,P〈0.01）.CONCLUSION The level of ENA-78 increases at blood neutrophil of rat asthma.Neutrophil may be participated in inflammation of asthma exacerbation via ENA-78.The asthmatic airway inflammation may decreased through reducing NEU ENA-78 by Budesonide.

关 键 词：哮喘 内皮中性粒细胞激活肽-78 免疫组织化学 中性粒细胞 布地奈德 

分 类 号：R557.3[医药卫生—血液循环系统疾病]