淋病患者支原体属合并感染研究  

Study on mycoplasmas causing coinfection in patients with gonorrhea

作　　者：李琴[1] 乔伟振[2] 张丽萍[1] 肖琮月[1] 陈国千[1] 

机构地区：[1]南京医科大学附属无锡市人民医院检验科,江苏无锡214023 [2]南京医科大学附属无锡市人民医院中心实验室,江苏无锡214023

出　　处：《中华医院感染学杂志》2011年第18期3960-3962,共3页Chinese Journal of Nosocomiology

摘　　要：目的了解男、女性淋病患者支原体属合并感染情况。方法应用套式PCR技术,对60例男性淋病患者的尿道拭子和42例女性淋病患者的子宫颈拭子,进行解脲脲支原体(Uu)、人支原体(Mh)、肺炎支原体(MP)、生殖支原体(MG)、发酵支原体(Mf)的检测,部分病例进行了DNA全自动测序。结果男性淋病患者>1种支原体合并感染率为41.67%,女性则达90.48%,后者明显高于前者(P<0.001);5种支原体中以Uu、Mh感染率最高,其中男性感染率分别为31.67%,16.67%,女性分别为71.42%,57.14%,后两者明显高于前两者,差异有统计学意义(P<0.01);男、女性均未发现合并有Mf感染者;Uu、Mg、Mp各1例套式PCR产物经直接DNA全自动测序,与标准株序列完全一致。结论男、女性淋病患者支原体属合并感染率较高,其中以Uu、Mh最为常见。OBJECTIVE To study the state of mycoplasmas causing coinfection in the patients with gonorrhea.METHODS Nested polymerase chain reaction（nPCR） was performed to detect nucleic acid of 5 kings of mycoplasmas including Ureaplasma urealyticum（Uu）,Mycoplasma hominis（Mh）,Mycoplasma pneumoniae（Mpn）,Mycoplasma genitalium（Mg） and Mycoplasma fermentans（Mf）,which were from 60 cases of urethra secretion swab sample tests on the male patients with gonorrhea and 42 cases of cervical secretion swab sample tests on the female patients with gonorrhea.The nucleic acid was sequenced by the automatic DNA sequencer to some positive cases.RESULTS In the patients with gonorrhea,the infectious positive rates were 41.67%（male） and 90.48%（female） by more than one kind of Mycoplasma,respectively.And the difference was significant between male and female（P〈0.01）.Among 5 kings of mycoplasma,the positive co-infection rates of Uu were 31.67%（male） and 71.42%（female）（P〈0.01）,and the positive co-infection of Mh were 16.67%（male） and 57.14%（female）（P〈0.01）,respectively.The PCR products of Uu,Mg and Mpn were sequenced,the results showed that the fragments were uniform with standard sequences.No merging Mf infection was found in the patients with gonorrhea.CONCLUSION The coinfection rate of Mycoplasma is higher among the patients with gonorrhea.Uu and Mh are the most common Mycoplasma in these patients.

关 键 词：淋病 支原体属 合并感染 

分 类 号：R375[医药卫生—病原生物学]