猪繁殖与呼吸综合征病毒GP5基因及猪IL-18基因共表达真核表达系统的建立  

Construction and identification of DNA vaccine co-expressing swine IL-18 and GP5 protein of porcine reproductive and respiratory syndrome virus

作　　者：李国江[1,2] 冯立文[1] 高磊[3] 丁壮[1] 张立春[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林农业科技学院,吉林吉林132101 [3]吉林农业大学动物科技学院,吉林长春130062

出　　处：《中国兽医学报》2011年第9期1241-1245,共5页Chinese Journal of Veterinary Science

基　　金：广东省科技攻关重大项目(2008A020100020)

摘　　要：采用RT-PCR方法扩增了JL/07/SW毒株GP5蛋白和猪IL-18基因。将该基因克隆入真核表达载体pEG-FP-N1,获得重组质粒pEGFP-GP5和pEGFP-IL18-GP5。将重组质粒转染Marc-145细胞,通过Western blotting和green fluorescent检测产物的表达情况。结果显示,所构建的2个重组质粒在Marc-145细胞中能进行有效的转录。结果表明,所构建的重组质粒pEGFP-GP5和pEGFP-IL18-GP5在Marc-145细胞中得到表达,为进一步研究PRRSV基因工程疫苗奠定了基础。Swine IL-18 and GP5 protein of the JL/07/SW strain of PRRSV was amplified using RT-PCR,and then cloned into eukaryotic expression vector pEGFP-N1 to construct recombinant plasmid pEGFP-GP5 and pEGFP-IL18-GP5,and then transfer to Marc-145 cells.Expression products were detected by Western blot-ting and green fluorescent.These two recombinant plasmid pEGFP-GP5 and pEGFP-IL18-GP5 were transcribed effectively and the objective proteins were expressed in Marc-145 cells.The recombinant expression vector pEGFP-GP5 and pEGFP-IL18-GP5 was successfully constructed,and object protein was expressed in Marc-145 cells.It laid the foundation of further study on genetic engineering vaccine of PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒 GP5 IL-18 核酸疫苗 

分 类 号：S852.65[农业科学—基础兽医学]