PCR检测猪输血传播病毒Ⅱ型方法的建立及其应用  

Development and application of the PCR for detection of swine torque teno virus genogroup Ⅱ

作　　者：娄高明[1] 冯顺胜[1] 殷华平[1] 黄健强[1] 

机构地区：[1]韶关学院动物疫病研究所,广东韶关512005

出　　处：《中国兽医学报》2011年第9期1259-1261,1289,共4页Chinese Journal of Veterinary Science

基　　金：广东省科技攻关项目(2007A020300006-5;2009B08-0800052);广东省高等学校自然科学研究重点项目(06Z024);广东省农业厅科技攻关项目(粤农函[2006]612号);韶关市技术创新项目(2008C/N01);韶关市人才基金项目

摘　　要：根据GenBank中猪源输血传播病毒Ⅱ型(Swine torque teno virus genogroupⅡ,TTVⅡ)基因序列设计并合成了1对特异性引物,扩增468bp目的片段。将PCR扩增产物测序,结果与GenBank中猪TTVⅡ型基因序列同源性为95%。利用该方法对猪圆环病毒Ⅱ型、猪细小病毒、猪伪狂犬病病毒、猪链球菌、猪多杀性巴氏杆、猪霍乱沙门菌和大肠杆菌等病原核酸进行扩增,均无条带出现,表明该方法具有较高的特异性。该方法的灵敏性试验表明,最低能检测到10.2pg核酸。利用所建立的PCR方法检测来自我国华南地区192份临床样品,阳性率为21.4%(41/192)。A pair of primers that amplified the gene of TTVⅡwas designed and synthesized.The PCR method was developed to detect swine torque teno virus genogroup Ⅱ（TTVⅡ）.The PCR product obtained was about 468 bp and shared 95% identity with the reported sequence.This result showed that the specific fragment was amplified in swine TTVⅡ,however,negative results were obtained from procine circovirusⅡ,procine parvovirus,procine pseudorabies,Streptococcus suis,Pasteurella multocida,Escherichia coli,salmonella choleraesuis.The sensitivity of the assay was found to be amplified 10.2 pg DNA.The PCR was applied to detect 192 samples from south China,and 21.4% samples（41/192） were swine TTVⅡ-positive.The result showed that established PCR technique provided a specific,sensitive and reliable method for detection and epizootic study of swine TTVⅡ.

关 键 词：猪输血传播病毒Ⅱ型 PCR 建立 应用 

分 类 号：S852.65[农业科学—基础兽医学] S854.43[农业科学—兽医学]