传染性支气管炎病毒感染对蛋雏鸡输卵管雌激素受体及孕激素受体表达的影响  

Influence of IBV on expression of estrogen receptor and progesterone recepto r in oviduct of layer chickens

作　　者：路洪岩[1] 李玉荣[1] 邹立宏 武现军[1] 霍书英[1] 赵国先[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北保定瑞普生物药业有限责任公司,河北保定071001

出　　处：《中国兽医学报》2011年第9期1283-1285,1308,共4页Chinese Journal of Veterinary Science

基　　金：河北省科技研究与发展规划资助项目(07220401D-9)

摘　　要：以不同传染性支气管炎病毒(肾型IBV-河北分离株与呼吸型IBV-M41)分别感染蛋雏鸡,通过测定输卵管蛋白分泌部及子宫雌激素受体(Estrogen receptor,ER)和孕激素受体(Progesterone receptor,PR)的表达水平,来探讨IBV早期感染(10d)对抑制蛋鸡输卵管发育的作用机制。结果显示,IBV感染降低子宫和蛋白分泌部ER mR-NA、PR mRNA的表达水平,即致子宫ER mRNA和PR mRNA的表达低于检测水平;蛋白分泌部ER mRNA和PR mRNA的表达水平显著降低(P<0.01),且M41的作用幅度较肾型IBV更为显著(P<0.01)。结果表明,IBV可通过降低ER和PR的表达水平,抑制输卵管对雌激素的应答能力而影响输卵管的发育。In order to study the effect of infectious bronchitis virus（IBV） on es trogen（E） and progesterone（P） receptors expression in the magnum and the shell gland of oviduct,their respective transcripts（EP mRNA and PR mRNA） were determ ined by RT-PCR,and two strains of IBV（M41 and a Hebei kidney-type strain） we re used.The results showed that IBV infection reduced the expression of ER and PR i n the magnum and the shell gland of oviduct.The expression of ER and PR was not detected in the shell gland of IBV infection groups,while in the contol it is ex pressed normal.The expression of ER and PR in the magnum was significantly decre aced（P 0.01）,and the role of M41 was more significant than the kidne y-type IBV（P0.01）.The study revealsed that IBV can affect the development of o viduct by reducing the expression of ER and PR,and so downgrade responsing capab ilities of the estrogen.

关 键 词：IBV 蛋雏鸡 输卵管 雌激素受体 孕激素受体 

分 类 号：S811.2[农业科学—畜牧学] S852.65[农业科学—畜牧兽医]