湖南猪源粪肠球菌的分离鉴定及16S rDNA系统进化分析  

Finding and 16S rDNA phyletic evolution analysis of Enterococcus faecalis isolated from swine in Hunan province

作　　者：黎满香[1] 林荣高[1] 薛立群[1] 蒋伟[1] 陈滔[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《中国兽医学报》2011年第9期1290-1294,共5页Chinese Journal of Veterinary Science

基　　金：湖南省科技计划资助项目(2010NK3015)

摘　　要：从湖南各地送检至实验室的临床样本中分离到42株革兰氏染色阳性及过氧化氢酶阴性的球菌,其中78.6%来自于肺脏,菌落形态及染色镜检均与粪肠球菌参考株ATCC 29212相似,兰氏分群鉴定97.6%(41/42)为D群,生化特性符合粪肠球菌特征。16SrDNA测序鉴定显示:42个分离株与同步测序的ATCC 29212同源性在99.2%到100%之间,与所选择粪肠球菌参考序列的同源性在98.7%到100%之间,NCBI在线BLAST分析发现42株均与GeneBank收录的粪肠球菌序列同源性最高。湖南分离株16SrDNA序列与E.faecalis参考序列进化关系与地域分布无关,来源于不同宿主的菌株的16SrDNA变异并不大,而与E.faecium、E.canis、E.avium、E.hirae等4种肠球菌则有多处变异,这些变异区域在这4种肠球菌中却是保守的。In this study,Gram＇s staining of positive and catalase negative coccus strains（n= 4Z） was isolated from clinleal diseased pig in Hunan province, most of those strains were from lung（78.6%）. The colonial morphology and the shape of Cram strain bear a close analogy to ATCC29212 strain. Laneefield test showed that 97.6%（41/42）strains belongs to group D. The sequence analysis of 16S rDNA revealed that all Hunan strains share a high identity with reference strain ATCC29212（99.2%）and the homology ranged from 98.7 ~ to 100 ~ when comparing with selected reference sequences. The 16S rDNA sequence of Hunan isolates had highest identity with E. faecalis published in GeneBank by BLAST in NCBI. The phyletic evolution of all compared E. faecalis strains have no conneetion with the geographic distribution and host, more variation was observed in the 16S rDNA between E. faecalis and other four Enterococcus , ineluding E. f aecium. E. canis . E. avium. E. hirae , but these variation region are conservative in the four species.

关 键 词：粪肠球菌 鉴定 16S RDNA 

分 类 号：S852.61[农业科学—基础兽医学]