堆型艾美耳球虫重组DNA质粒pcDNA3-3-1E的免疫原性  

Immunogenicity of recombinant DNA plasmid pcDNA3-3-1E of Eimeria acervulina

作　　者：常丽云[1] 赵月兰[2] 刘义伟[1] 张丽娟[1] 秦建华[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北农业大学中兽医学院,河北定州073000

出　　处：《中国兽医学报》2011年第9期1301-1303,共3页Chinese Journal of Veterinary Science

基　　金：河北省自然科学基金资助项目(C2007000523);国家自然科学基金资助项目(30571394)

摘　　要：用E.acervulina重组质粒pcDNA3-3-1E免疫雏鸡,观察其对雏鸡细胞免疫和体液免疫功能的影响。将7日龄雏鸡随机分为4组,即空白对照组、pcDNA3.1空质粒组、重组质粒免疫组和卵囊免疫组,每组30只。pcDNA3-3-1E经肌肉注射途径免疫雏鸡,剂量为50μL/只(含质粒1g/L)。7日龄免疫1次,14日龄加强免疫1次。检测雏鸡血液T淋巴细胞增殖率,测定血清抗体水平。结果显示,与空质粒组和健康对照组相比,重组质粒组淋巴细胞的增殖能力明显增强(P<0.05),外周血中IgG的含量均显著升高(P<0.05),表明pcDNA3-3-1E裸质粒DNA能有效的提高雏鸡的细胞免疫和体液免疫水平。The chickens were immuned with E.acervulina plasmid pcDNA3-3-1E to observe the function of the cellular and humoral immune.The 7 day-old chickens were randomly divided into 4 groups,which was control group,pcDNA3.1 empty plasmid group,recombinant plasmid immunization group,oocyst immune group,and every group contained 30 chickens.The chickens were immued with pcDNA3-3-1E by intramuscular route at a dose of 50 μL/per（including plasmid 1 g/L）,and strengthened immunity one week later.The blood T lymphocyte proliferation,serum antibody levels were detected.The results showed that the lymphocyte proliferation of recombinant plasmid group significantly increased（P0.05） and the peripheral blood IgG were significantly higher（P0.05） than those of the empty plasmid group and the healthy control group.The pcDNA3-3-1E naked plasmid DNA can effectively improve the cellular and humoral immune responses of the chickens.

关 键 词：堆型艾美耳球虫 质粒pcDNA3-3-1E 免疫原性 

分 类 号：S852.7[农业科学—基础兽医学]