快速检测与种公牛精液品质相关的FSHβ基因连锁突变方法的建立  

A rapid method to detect the multiple SNPs in FSHβ exon 3 with relation to semen quality and fertility in bulls

作　　者：于汪洋[1,2] 许艳丽[1,2] 戴立胜[1,2] 袁宝[2] 高妍[2] 马腾壑[1,2] 姜昊[1,2] 熊秋宏[1,2] 邓琼[1,2] 赵锐锋 张嘉保[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062 [3]吉林省长春优质肉牛繁育中心,吉林长春130000

出　　处：《中国兽医学报》2011年第9期1358-1361,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972100);国家肉牛牦牛产业技术体系(nycytx-38)

摘　　要：通过PCR克隆测序方法和TaqMan-MGB探针基因分型方法分别对86例样本中FSHβ第三外显子(4 338T→C、4 341C→T、4 350G→A)3个位点的连锁突变进行检测。结果表明:2种方法所得结果完全一致,AA型基因频率显著高于AB型(P<0.05)。本试验建立的TaqMan-MGB探针快速检测与种公牛精液品质相关的FSHβ基因连锁突变的方法,为种公牛的早期选种选育提供了技术支撑。In 86 samples,FSHβ exon 3（4 338T→C,4 341C→T and 4 350G→A） multiple SNPs was analyzed by cloning,DNA sequencing and TaqMan-MGB probes genotyping method.The sequencing results indicated that these results of the two methods were completely identical,and AA genotype frequency was higher than AB genotype（P0.05）.A rapid method to detect multiple SNPs of the FSHβ gene with relation to semen quality and fertility in bulls,and the research laid the foundation for the future studies of the technological support for breeding of bulls.

关 键 词：单核苷酸多态性 促卵泡素β 实时定量PCR TAQMAN-MGB探针 

分 类 号：S814[农业科学—畜牧学]