阳离子脂质体基因转染研究  

Study on Gene Transfection Mediated by Cationic Liposomes

作　　者：韩维奇[1] 关山飞[1] 朴林波[1] 梁超[1] 张树彪[1] 王冰[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600

出　　处：《广东化工》2011年第9期36-36,64,共2页Guangdong Chemical Industry

摘　　要：目的:评价阳离子脂质体介导基因转染宫颈癌细胞的转染效果,优化对宫颈癌细胞的转染条件。方法:以荧光素酶基因pGL3为报告基因,分析影响阳离子脂质体Lipofectamine 2000转染Hela效率的因素。结果:通过细胞转染实验,得出脂质体与pDNA的稀释液混合较佳等待时间为30 min,脂质体/pDNA复合物与细胞接触的转染时间为4 h,转染效率较高。结论:Lipofectamine2000 Hela细胞转染效率较高。Objective： To evaluate the gene transfection efficiency of cationic liposome to cervical carcinoma cell,in addition,to optimize the transfection conditions.Method： the transfection efficiency of liposome were examined using Luceferase as reporter gene.Result： Lipofectamine 2000 could get higher transfection efficiency when dilutin time was 30 min before complexing and the optimum transfection time was 4 h.Conclusion： Lipofectamine 2000 obtained higher transfectin efficiency in Hela cells.

关 键 词：阳离子脂质体 基因转染 报告基因 

分 类 号：TQ[化学工程]