黄垂网不同发育时期肌动蛋白基因的序列分析  

Analysis of Sequences of Arcyria obvelata in Different Period Based on Actin Gene

作　　者：陈小姝[1] 谷硕[1] 王琦[1] 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118

出　　处：《安徽农业科学》2011年第24期14538-14539,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(Nos.70670015&71070012)

摘　　要：[目的]对黄垂网不同发育时期(幼孢囊和成熟孢囊时期)的肌动蛋白基因的序列进行测定、比对。[方法]基于肌动蛋白基因,利用分子生物学方法对黄垂网不同发育时期的DNA进行提取、测序。[结果]不同发育时期的序列同源性在99%以上,具有的氨基酸数量相同,都为19种,没有色氨酸。丝氨酸含量最高,组氨酸含量最低。[结论]除丙氨酸、苯丙氨酸、异亮氨酸和脯氨酸外,黄垂网在不同发育时期的氨基酸含量均相同。[Objective]The sequences of Arcyria obvelata in different development periods were sequenced and contrasted based on actin gene.[Method] DNA of young and mature A.obvelata was exacted and sequenced by molecular methods based on actin gene.[Result] The homology of the sequences of young and mature A.obvelata was above 99%,and the numbers of amino acid were the same.Among 19 kinds of amino acid,there was no Trp.The content of Ser was the highest,and the content of His was the lowest.[Conclusion] The amino acid content of young and mature A.obvelata was the same except Ala,Phe,Ile and Pro.

关 键 词：黏菌 黄垂网 不同发育时期 肌动蛋白基因 

分 类 号：S188.1[农业科学—农业基础科学]