小球藻对3种常用抗生素的敏感性研究  

Study on the Sensitivity of Chlorella sp.to Three Common Antibiotics

作　　者：麻晓霞[1] 石勋祥[1] 马丽萍[1] 马玉龙[1] 

机构地区：[1]宁夏大学化学化工学院,宁夏银川750021

出　　处：《安徽农业科学》2011年第25期15199-15201,共3页Journal of Anhui Agricultural Sciences

基　　金：宁夏高等学校科学研究项目;国家大学生创新实验项目(11HGG04);宁夏大学大学生创新实验项目(11HGG04)

摘　　要：[目的]探讨小球藻(Chlorellasp.)对3种常用抗生素的敏感性。[方法]采用分光光度法研究了青霉素、氨苄青霉素、头孢他啶3种常用抗生素对小球藻生长的影响,以确定分离、纯化微藻时对藻细胞无害并能抑制伴生杂菌生长的抗生素浓度。[结果]小球藻对青霉素较为敏感,而对氨苄青霉素和头孢他啶敏感性较弱,低浓度(1.0 mg/L)的氨苄青霉素对微藻细胞生长有明显促进作用。[结论]氨苄青霉素可用于小球藻纯化过程中抑菌,其适宜浓度为1.0 mg/L。[Objective] The aim was to esplore the sensitivity of Chlorella sp.to 3 kinds of common antibiotics.[Method] Effects of three common antibiotics such as penicillin,ampicillin,and ceftazidime on the growth of Chlorella sp.were studied by spectrophotometric method.The aim was to determine the optimal antibiotic concentration,which was harmless to the growth of Chlorella sp.,but had an inhibitory action on bacteria.[Result] Penicillin had strong inhibition to the Chlorella sp.while ampicillin and ceftazidime exhibited a low inhibition.An amount of 1.0 mg/L of ampicillin significantly promoted the growth of the tested microalgae.[Conclusion] Ampicillin can be used to prevent microbial contamination during the purification of Chlorella sp..The optimal content of ampicillin was 1.0 mg/ L.

关 键 词：小球藻 抗生素 敏感性 生长抑制 

分 类 号：S132[农业科学—农业基础科学]