海南木莲叶和枝总异黄酮的含量测定  

Determination of Isoflavones in the Leaves and Stems of Manglietia hainanensis

作　　者：鲍长余[1] 范超君[1] 陈湛娟[1] 毕和平[1,2] 

机构地区：[1]海南师范大学化学与化工学院,海南海口571158 [2]海南师范大学"省部共建-热带药用植物化学"教育部重点实验室,海南海口571158

出　　处：《安徽农业科学》2011年第25期15270-15271,15274,共3页Journal of Anhui Agricultural Sciences

基　　金：海南省教育厅资助项目(Hjkj2008-27);海南师范大学大学生创新实验项目(081165814)

摘　　要：[目的]测定海南木莲叶和枝中总异黄酮的含量。[方法]以染料木素为对照品,采用紫外分光光度法在266 nm处测定样品的吸光度。[结果]染料木素对照品在20~100μg时呈良好线性关系。其回归方程为C=0.003 9A,R=0.999 9。海南木莲叶、枝中总异黄酮的含量为0.240%和0.078%。[结论]该方法操作简便,结果稳定,重复性好,适合海南木莲叶和枝中总异黄酮含量的测定。[Objective] The aim was to determine the content of the isoflavones in the leaves and the stems of Manglietia hainanensis.[Methods] Genistein was viewed as reference substance,the ultraviolet spectrophotometric method was used to determine the content of the isoflavones in the leaves and branches of Manglietia hainanensis.[Results]The calibration curve was linear in the range of 0.000 5-0.004 5 μg/ml,and the regression equation was C=0.003 9A,R=0.999 9,the content was 0.240 % and 0.078 %.[Conclusion] The spectrophotometry was suitable for the determination of isoflavones from the leaves and branches of Manglietia hainanensis.This method operated conveniently,the result was stable and has good repeatability.

关 键 词：紫外分光光度法 海南木莲 总异黄酮 

分 类 号：S567[农业科学—中草药栽培]