半夏疏松愈伤组织诱导条件的优化研究  

Study on the Induced Condition Optimization of Pinellia ternata Loose Callus

作　　者：朱艳芳[1] 毛春娜[1] 张爱民[1] 薛建平[1] 李佳娣[1] 宋运贤[1] 

机构地区：[1]淮北师范大学生命科学学院资源植物生物学安徽省重点实验室,安徽淮北235000

出　　处：《安徽农业科学》2011年第25期15275-15276,15292,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30973963);安徽省自然科学基金项目(090413252);安徽高校省级自然科学研究重点项目(KJ2009A160);资源植物生物学重点实验室项目(KLPRB200914)

摘　　要：[目的]建立半夏细胞悬浮培养体系。[方法]以半夏叶柄为外植体,采用正交试验设计研究了2,4-D、NAA、毒莠定和KT 4种植物生长物质及其配比对疏松愈伤组织形成的影响。[结果]2,4-D和毒莠定对半夏叶柄愈伤组织诱导效果最显著,KT和NAA次之。诱导半夏叶柄形成疏松愈伤组织的适宜培养基为MS+0.5 mg/L 2,4-D+1.0 mg/L NAA+1.0 mg/L毒莠定+1.5 mg/L KT。[结论]为通过半夏细胞悬浮提取有效成分和生产人工种子奠定了基础。[Objective] The research aimed to establish the cell suspension culture system of Pinellia ternate.[Method] The petiole of Pinellia ternate was as the explant.The orthogonal experiment was used to design and study the influences of four plant growth substances（2,4-D,NAA,picloram and KT） and their mixture ratios on the formation of loose callus.[Result] The induction effects of 2,4-D and picloram on the petiole callus of Pinellia ternate were the most significant.Then,the second ones were KT and NAA.The optimal medium which induced the petiole of Pinellia ternate to form the loose callus was MS ＋ 0.5 mg/L 2,4-D ＋ 1.0 mg/L NAA ＋ 1.0 mg/L picloram ＋ 1.5 mg/L KT.[Conclusion] The research laid the foundation for extracting the active ingredient from the cell suspension of Pinellia ternate and producing the artificial seed.

关 键 词：半夏 叶柄 疏松愈伤组织 悬浮培养 

分 类 号：S132[农业科学—农业基础科学]