2种中药提取物对番茄灰霉病菌和番茄早疫病菌的抑制活性研究  

Antifungal Activity of Botanical Extracts against Botrytis cinerea and Alternaria Solani

作　　者：毕亚玲[1] 王波[1] 黄保宏[1] 张文同[1] 张轶辉[1] 

机构地区：[1]安徽科技学院植物科学学院,安徽凤阳233100

出　　处：《安徽农业科学》2011年第25期15309-15310,15313,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽科技学院引进人才基金项目(ZRC2007102);安徽省高校优秀青年人才基金项目(2009SQRZ111)

摘　　要：[目的]研究白术和白头翁2种中药提取物的杀菌活性,为其在植物源农药上的开发利用奠定基础。[方法]采用菌丝生长速率法,研究了白术和白头翁2种植物提取物对番茄灰霉病菌和番茄早疫病菌的抗菌活性。[结果]供试2种中药提取物对番茄灰霉病菌和番茄早疫病菌均具有较好的抗菌活性,其中白头翁提取物的抗菌活性最好,在供试提取物质量吸度为10 mg/ml时,72 h白头翁正丁醇提取物对番茄灰霉病菌的抑制率达80.25%,白术石油醚提取物对番茄灰霉病菌的抗菌活性较好,抑制率为71.36%,且提取物的抗菌活性随着吸度的增大而增强,白头翁正丁醇提取物对番茄灰霉病菌的EC50为2.93 mg/ml,白术石油醚提取物对番茄灰霉病菌的EC50为5.31 mg/ml。[结论]白头翁提取物对番茄灰霉病菌和番茄早疫病菌的抑制活性较好,值得进一步研究。[Objective] The antifungal activity of the extracts from Atractylodes macracephal Koidz and Pulsatilla chinensis Bunge Regel,against Botrytis cinerea and Alternaria solani were studied under the condition of laboratory,in order to develop and utilize these two plants.[Method] The mycelium growth rate test was applied to measure the antifungal activities of extracts against fungi.[Result] The extracts of all the two plants showed strong antifungal activity against the target pathogenic fungi,especially the antifungal activity of the extract from Pulsatilla chinensis Bunge Regel was stronger and more stable.The inhibition rate to the mycelium growth of Botrytis cinerea was 80.25% and the EC50 was 2.93 mg/ml.At the same concentration,the extract from Atractylodes macracephal Koidz showed little inhibition to Botrytis cinerea and Alternaria solani,the inhibition rate to the mycelium growth of Botrytis cinerea was 71.36% and the EC50 was 5.31 mg/ml.[Conclusion] The extracts from Pulsatilla chinensis Bunge Regel showed the stronger antifungal activity against Botrytis cinerea and Alternaria solani.

关 键 词：提取物 抗菌活性 番茄灰霉病菌 番茄早疫病菌 

分 类 号：S436.412.13[农业科学—农业昆虫与害虫防治]