龙爪槐提取物对铜绿微囊藻生长的抑制作用研究  

Study on the Inhibitory Effect of Sophora japonica Extracts against the Growth of Microcystis aeruginosa

作　　者：周晓见[1,2] 夏洁[1] 靳翠丽[1,2] 缪莉[1,2] 董昆明[1,2] 封克[1,2] 

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]扬州大学海洋科学与技术研究所,江苏扬州225127

出　　处：《安徽农业科学》2011年第25期15322-15324,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(41076097;41006097);教育部科学技术研究重点项目(211065);江苏省自然科学基金项目(BK2010322);江苏省环境材料与环境工程重点实验室开放课题(K090027;K090025;K090026;K090028);江苏省教育厅研究生科技创新项目(M080960);扬州大学"新世纪"人才工程项目

摘　　要：[目的]探讨龙爪槐提取物对铜绿微囊藻生长的抑制作用。[方法]测定了龙爪槐叶片浸取液对铜绿微囊藻的增殖抑制效果,并对活性组分进行了初步研究和分析。[结果]龙爪槐叶片的无水乙醇浸提物经正己烷、乙酸乙酯、正丁醇和水依次分配后,提取物主要成分为极性组分(60%以上),而含量约为25%的非极性组分(正己烷相)具有显著的抑制铜绿微囊藻生长的效果。在25和50 mg/L正己烷相组分作用下铜绿微囊藻的7 d生长抑制率分别达75%和90%以上,同时还伴随着细胞中叶绿素a的破坏。[结论]为防治微囊藻水华提供了理论依据。[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of Microcystis aeruginosa was measured.Moreover,the active component was studied and analyzed initially.[Result] The absolute alcohol extract of Sophora japonica leaf was separated to n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority（60%）.The non-polar fraction in n-hexane（about 25%） was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.[Conclusion] The research provided the theory basis for preventing and controlling the water bloom of M.aeruginosa.

关 键 词：龙爪槐 铜绿微囊藻 正己烷相 叶绿素A 

分 类 号：X524[环境科学与工程—环境工程]