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作 者:陈曲[1] 翟慧者[1] 胡同乐[1] 王树桐[1] 曹克强[1]
机构地区:[1]河北农业大学植物保护学院,河北保定071000
出 处:《安徽农业科学》2011年第25期15337-15338,15351,共3页Journal of Anhui Agricultural Sciences
基 金:国家苹果现代产业技术体系项目(nycytx-08-04-01);公益性行业(农业)科研专项经费项目(200903004-42)
摘 要:[目的]为了给生产实践中苹果树腐烂病研究提供一种获得分生孢子的方法。[方法]在室内测试苹果树腐烂病菌在6种培养基上的分生孢子产生条件,比较每种培养基上产生的分生孢子器个数、密度、大小、成熟率以及单个分生孢子器所含孢子数量等。[结果]PDA(添加马铃薯)、ABA(添加苹果树皮)培养基在各项参数对比中显著优于其他培养基。其中,PDA上产生的分生孢子器数量最多,ABA上产生的分生孢子器成熟率最高,产生成熟子实体的时间最短;黑光灯照射(紫外照度为53.5μW/cm2)有利于苹果树腐烂病菌分生孢子器的产生和成熟。[结论]提供了一种较为方便的室内获得苹果树腐烂病孢子的方法。[Objective] The method of the formation of the conidiospore of apple tree canker[Valsa ceratosperma(Tode et Fr.) Marie] was provided for the research on it in actual production.[Method] The formation condition of the spore of apple tree canker pathogen cultured in six media inside room was experimented and the number,density,size,rate of maturity of pycnidia and the number of spore per pycnidia in each medium were compared.[Result] It was indicates that all of parameters in PDA(added with potato) and ABA(added with apple tree bark were better than those of others.The most number of conidiocarp was produced in the PDA and the highest rate of maturation conidiocarp was formed in the ABA,the formation time of which mature fruiting body was shortest.The black light lamp(53.5 μW/cm2 of UV radiation) was beneficial to the growth and maturity of conidiocarp.[Conclusion] One kind of convenient approach of spore formation of apple tree canker indoor was provided.
分 类 号:S432[农业科学—植物病理学]
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