Cu^(2+)对蚯蚓和家兔AChE毒性研究  

Study on Toxic Effect of Cu2+ on Acetylcholinesterases(AChE) of Two Living Things

作　　者：张轶辉[1] 崔小伟[2] 毕亚玲[1] 王波[1] 

机构地区：[1]安徽科技学院植物科学学院,安徽凤阳233100 [2]河南省农业科学院植物保护研究所,河南郑州450002

出　　处：《安徽农业科学》2011年第25期15342-15344,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽科技学院引进人才项目(zrc2008203)

摘　　要：采用体外试验方法,以蚯蚓和家兔为研究对象,分别测定了Cu2+对二者AChE的半抑制浓度(IC50)和双分子速率Ki。结果表明,在抑制时间为10 min时Cu2+对蚯蚓AChE和家兔脑部AChE的IC50分别为3.6和2.4 mmol/L;在IC50浓度的Cu2+作用下,Cu2+对家兔脑部AChE的Ki值较大,为Cu2+对蚯蚓AChE的3.59倍,表明家兔脑部AChE较蚯蚓AchE对Cu2+更为敏感。Effects of Cu2＋ on acetylcholinesterase（AChE） of habit brain and earthworm was evaluated in vitro.Results showed that AChE activities were depressed significantly by concentration ranges of Cu2＋ respectively.The IC50 values of Cu2＋ were 3.6 and 2.4 mmol/L,respectively.Under the function of Cu2＋ with IC50 concentration,Ki value of AChE from habit brain was 3.59 times of that of earthworm,this show that AChE from habit brain had higher sensitivity to Cu2＋ than that of earthworm.

关 键 词：ACHE CU2+ 毒性 

分 类 号：S154[农业科学—土壤学]