检测H9亚型AIV的压电免疫传感器的研究  

Study on the Piezoelectric Immunosensor for the Detection of H9 Subtype Avian Influenza Virus

作　　者：刘靖清 卞红春 胡云发[2] 王新卫[3] 陈枝楠[2] 詹爱军[2] 

机构地区：[1]盐城市农业委员会,江苏盐城224000 [2]深圳市检验检疫科学研究院,广东深圳518010 [3]河南农业大学牧医工程学院,河南郑州450002

出　　处：《安徽农业科学》2011年第25期15384-15386,共3页Journal of Anhui Agricultural Sciences

基　　金："十一五"国家科技支撑项目(2006BAK20A29);广东省科技重大专项计划项目(2004A2090102)

摘　　要：[目的]研制检测H9亚型禽流感病毒的压电免疫传感器。[方法]用巯基丙酸在镀银电极石英晶体自组装巯基丙酸单分子膜再通过N-乙基-N-′（3-二甲氨基）丙基碳化二亚胺盐酸（EDC）和N-羟基琥珀酰亚胺（NHS）偶联抗H9亚型禽流感病毒的特异性单抗构建传感器芯片,建立可以检测H9N2亚型禽流感病毒的免疫传感器。[结果]该方法具有较好的特异性,不与H5亚型流感病毒和新城疫病毒（NDV）反应;检测灵敏度达到20~100EID50。[结论]该研究为深入研究检测禽流感病毒的免疫传感器奠定了基础,为其他相关病毒的监测提供了一种新途径。[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor was set up by self-assembling mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N′-（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）.The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other viruses in animal husbandry.

关 键 词：压电免疫传感器 生物自组装法 H9亚型禽流感病毒 

分 类 号：S855.3[农业科学—临床兽医学]