野田村病毒RNA的复制机制  

RNA replication mechanism of nodaviruses

作　　者：刘传凤[1] 张珈敏[2,3] 胡远扬[2,3] 

机构地区：[1]武汉纺织大学环境工程学院,湖北武汉430073 [2]武汉大学生命科学院病毒学国家重点实验室 [3]环球生物农药武汉大学联合研发中心,湖北武汉430072

出　　处：《微生物学通报》2011年第9期1418-1424,共7页Microbiology China

摘　　要：野田村病毒科Nodaviradae分为2个属,分别为主要感染昆虫的α野田村病毒属(Alphanodavirus)和主要感染鱼类的β野田村病毒属(Betanodavirus)。野田村病毒的基因组由2条单链正义RNA分子(RNA1和RNA2)所组成,RNA1编码蛋白A,即病毒负责复制病毒两条基因组的依赖RNA的RNA聚合酶催化亚基。RNA2编码衣壳前体蛋白α,此前体蛋白α先组装成原病毒粒子,再经历一次自我催化的成熟切割成2个病毒的衣壳蛋白β和γ,就成了成熟的有感染性的病毒粒子。在RNA复制过程中,从RNA1的3′末端会合成一个不被包装进病毒粒子的亚基因组RNA3。RNA1能在无RNA2的情况下自我复制,并持续地产生亚基因组RNA3,RNA3的合成采取的是提前终止机制。本文还介绍了野田村病毒复制的调节、非结构蛋白的功能和病毒复制在细胞内的定位。The family Nodaviridae contains two genera,Alphanodaviruses and Betanodaviruses,which predominantly infect insects and fish,respectively.The genome of nodaviruse consists of two single-strand positive-sense RNAs（RNA1 and RNA2）.RNA1 encodes protein A,catalytic subunit of RNA-dependent RNA polymerase（RdRp）,and RNA2 encodes coat precursor protein which undergoes an autocatalytic mature cleavage into two viral capsid proteins β and γ.During the course of RNA replication,a sub-genome RNA3 is synthesized which is not packaged into the virion from the 3＇ termini of RNA1.RNA1 can self-replicate automatically absence of RNA2 and produce the sub-genome RNA3 persistently.The mechanism of RNA3 synthesis is the mechanism of premature termination.The paper also reviewed the regulation of RNA replication,the functions of non-structural proteins and the localization of RNA replication of the nodaviruses.

关 键 词：野田村病毒 基因组结构 复制机制 复制调节 复制的定位 

分 类 号：Q939.4[生物学—微生物学]