紫色非硫细菌固体石蜡双层平板培养法  

The paraffin wax overlay plate method for purple nonsulfur bacteria

作　　者：冯有智[1,2] 林先贵[1,2] 王一明[1,2] 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏南京210008 [2]中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,江苏南京210008

出　　处：《微生物学通报》2011年第9期1435-1442,共8页Microbiology China

基　　金：国家自然科学基金项目(No.41001142;40771202)

摘　　要：紫色非硫细菌是一类不产氧光合细菌,具有重要的生态学意义和应用价值。建立固体石蜡双层平板法,与传统的培养法相比较,发现该方法可以更方便、更快捷地计数和分离紫色非硫细菌。结合分子生物学技术,将该方法应用于水稻土紫色非硫细菌的研究,发现以甲酸为碳源的固体石蜡双层平板法可以很好地对环境中可培养的紫色非硫细菌多样性进行研究。该方法的建立将更有助于紫色非硫细菌的研究。Purple nonsulfur bacteria are one branch of purple phototrophic bacteria,which are ecologically important and have valuable applications.In this study,we established paraffin wax overlay plate method for the isolation and the enumeration of purple nonsulfur bacteria.Compared with several traditional methods,the new one was found to be more rapid and convenient.We applied this method together with molecular approaches to investigate purple nonsulfur bacteria in paddy soil,and found that it can well reveal the diversity of purple nonsulfur bacteria at culturable level,when formate acts as carbon source.We believe the new method would be of great help to the investigation of purple nonsulfur bacteria in environment.

关 键 词：紫色非硫细菌 可培养方法 水稻土 

分 类 号：Q93-3[生物学—微生物学]