环境因子对小球藻生长的影响及高产油培养条件的优化  

Effect of environmental factors on growth of Chlorella sp.and optimization of culture conditions for high oil production

作　　者：丁彦聪[1] 高群[1] 刘家尧[1] 衣艳君[1] 刘建国[2] 林伟[2] 

机构地区：[1]青岛农业大学生命科学学院,青岛266109 [2]中国科学院海洋研究所,青岛266071

出　　处：《生态学报》2011年第18期5307-5315,共9页Acta Ecologica Sinica

基　　金：国家重点基础研究发展(973)计划课题(2011CB200904);国家高技术研究发展(863)计划课题(2008AA09Z403);青岛农业大学人才基金项目(630744);青岛市科技计划基础研究项目(10-3-4-5-3-jch)

摘　　要：探讨了不同环境条件对小球藻(Chlorella sp.)叶绿素荧光动力学参数以及净光合放氧速率的影响,确定了以L1海水培养基为基础,以8.8 mmol/L浓度的(NH2)2CO为氮源、0.145 mmol/L NaH2PO.4H2O浓度为磷源,在150μmol.m-.2s-1光照强度、培养温度为18℃的小球藻最优培养条件。在此条件下,明显加快了小球藻细胞的生长速度,促进了油脂和脂肪酸的积累,细胞密度增加24%,油脂和脂肪酸含量分别增加了16.8%和66.6%。在培养液中添加外源柠檬酸(最适浓度以0.06 mmol.L-.1d-1为宜)可以明显提高小球藻的生长速度,促进其脂肪酸的积累。同时也可看出,筛选的小球藻藻种具有生长快、易培养、产油高的优点,可作为生物能源研究的良好材料,为海洋微藻的开发利用奠定了基础。After series of screening a marine chlorella was selected for further study.In L1 seawater medium the chlorophyll fluorescence induction dynamics of Chlorella sp.under different environmental conditions was analyzed after 48 hours.The results showed that the chlorophyll fluorescence induction dynamics parameters such as maximum quantum yield（Fv/Fm）,per excited cross section（CS） for trapping（TRo/CSo）,electron transport（ETo/CSo）,and density of active reaction centers（RC/CSo） of Chlorella sp.were affected by light intensity,temperature,and the concentration of nitrogen and phosphorus.The chlorophyll fluorescence parameters was increased with certain environmental factors,but inhibited when the environmental factors exceeded the limits.The best condition for chlorophyll fluorescence induction dynamics parameters of Chlorella was composed of 150μmol·m-2·s-1 light intensity,18℃ temperature,8.8mmol/L NaNO3,and 0.145mmol/L NaH2PO4·H2O.The net photosynthetic oxygen evolution rate of Chlorella sp.under different environmental conditions was examined,and the results were consistent with that of the chlorophyll fluorescence induction dynamics.Based on the formula of L1 medium,the optimal culture media of Chlorella sp.was determined（8.8mmol/L（NH2）2CO,0.145mmol/L NaH2PO4·H2O,150μmol·m-2·s-1 light intensity,18℃ temperature）.Under the optimized culture conditions,the cell density of Chlorella sp.was significantly higher than the conditions before optimization,the cell density reached 6.3×107/mL after 10 days culture,and the density was increased by 24%.The total lipid and fatty acid of Chlorella sp.were increased by 16.8% and 66.6% respectively.In addition,the cell growth rate and the accumulation of fatty acids of Chlorella sp.were improved by addition of exogenous citric acid（the optimal concentration was 0.06mmol·L-1·d-1）.Statistics analysis showed that the fatty acids content of Chlorella sp.was positively correlated with citric acid（P0.5）.Under the conditions of optimized l

关 键 词：小球藻 环境因子 细胞生长 脂肪酸 优化培养 

分 类 号：S94-3[农业科学—水产养殖]