SiO_2纳米粒子在毛细管电泳分离蛋白质中的应用  

Application of SiO2 Nanoparticles in Separation of Protein by Capillary Electrophoresis

作　　者：仲淑贤[1] 王卫平[1] 陈建荣[1] 

机构地区：[1]浙江师范大学化学与生命科学学院

出　　处：《光谱实验室》2011年第5期2460-2464,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：将SiO2纳米粒子作为添加剂用于溶菌酶、牛血清白蛋白、牛血红蛋白的毛细管电泳(CE)分离研究。对影响3种蛋白质分离的因素如缓冲溶液的浓度,SiO2纳米粒子、聚合物PVP、乙腈的含量,及分离电压、温度等进行了考察,得到最佳分离条件,即50mmol/L NaH2PO4-10mmol/L H3PO4缓冲溶液(pH=2.8),15%乙腈,10%PVP,0.35%SiO2,分离电压30kV,分离温度25℃,检测波长214nm时,3种蛋白质能得到很好的分离。结果表明,SiO纳米粒子是改善蛋白质毛细管电泳分离的有效添加剂。Separation of lysozyme,bovine serum albumin,bovine hemoglobin was studied by capillary electrophoresis（CE） with SiO2 nanoparticles as additives.The factors affecting separation,such as buffer concentration,the contents of additives（SiO2 nanoparticles,Polymer PVP,ACN）,separation voltage and temperature were investigated.Three proteins can be well separated under the optimum conditions of 50mmol/L NaH2PO4-10mmol/L H3PO4（pH=2.8）,15% ACN,10% PVP,0.35% SiO2,and an applied voltage of 30kV,temperature of 25℃,detection wavelength of 214nm.SiO2 nanoparticles could be used as effective additives for the separation of protein.

关 键 词：毛细管电泳 SIO2纳米粒子 溶菌酶 牛血清白蛋白 牛血红蛋白 

分 类 号：O657.8[理学—分析化学]