纤维素衍生物手性固定相HPLC拆分非洛地平对映体  

Separation of Enantiomer of Felodipine on Cellulose Derivatives Chiral Stationary Phase by HPLC

作　　者：唐晓丹[1] 王绍艳[1] 丛景香[1] 王智超[1] 

机构地区：[1]辽宁科技大学化学工程学院

出　　处：《光谱实验室》2011年第5期2573-2577,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：辽宁省教育厅创新团队项目(2008T093);辽宁科技大学青年基金团队项目子课题(2010T06);辽宁科技大学大学生科研训练项目(第六期)

摘　　要：采用高效液相色谱法(HPLC),在硅胶涂敷纤维素三-(4-甲基苯甲酸酯)手性固定相上拆分非洛地平。研究了异丙醇体积含量、流速及柱温对非洛地平对映体拆分行为的影响。同时还模拟了在制备分离条件时,增大进样体积和进样浓度对非洛地平拆分的影响。并结合色谱理论和热力学理论对非洛地平的手性识别机理进行探讨。实验表明在正己烷∶异丙醇=90∶10,流速0.8mL/m in,柱温30℃时,非洛地平对映体可实现基线分离。Chiral separation of felodipine was studied by high performance liquid chromatography on the chiral stationary phase of silica gel coated with cellulose 3-（4methylbenzoate）.The influences of the volume of isopropanol,flow rate and column temperature on the separation of felodipine were investigated.And the influences of increasing the injection volume and sample concentration on the separation of felodipine were also studied by simulating the preparation and separation conditions.The chiral recognition mechanism of felodipine was discussed by the chromatographic theory and thermodynamic theory.The baseline separation of felodipine was available in the mobile phase of hexane/isopropanol（90∶10） with a flow rate of 0.8mL/min and the column temperature of 30℃.

关 键 词：高效液相色谱法 纤维素衍生物固定相 非洛地平 手性分离 

分 类 号：O657.72[理学—分析化学]