广西龙荔遗传多样性的ISSR分析  

Genetic diversity analysis of Dimocarpus confinis in Guangxi by intersimple sequence repeats(ISSRs)

作　　者：潘丽梅[1,2,3] 朱建华[4] 秦献泉[4,3] 彭宏祥[4] 黄凤珠[4] 卢美英[1] 

机构地区：[1]广西大学农学院,南宁530005 [2]广西药用植物园,南宁530023 [3]广西作物遗传改良生物技术重点实验室,南宁530007 [4]广西农业科学院园艺研究所,南宁530007

出　　处：《果树学报》2011年第5期814-819,共6页Journal of Fruit Science

基　　金：广西农业科学院基本科研业务专项(201035基);广西科学基金项目(桂科自0339023)

摘　　要：选用l2条ISSR引物组合分析了广西67个龙荔单株和10个龙眼品种（单株）、12个荔枝品种以及l0个野生荔枝单株的遗传多样性,均得到了清晰指纹。在所扩增的151条ISSR主带中,有127条具有多态性,多态性比率占84.11%。所用引物可将供试的品种（单株）区分开来。供试龙荔单株的遗传相似系数在0.61~0.97,表明广西龙荔种质具有较广泛的遗传多样性。龙荔与龙眼、荔枝间遗传物质存在明显的差异,99份供试材料间的相似系数变化在0.53~0.98,在相似系数0.53处荔枝供试材料自聚为1类,而龙荔与龙眼聚为1类,表明龙荔与荔枝的遗传差异较大,龙荔在遗传物质上更接近龙眼。Twelve pairs of ISSR primer combinations were used to determine the genetic diversity of Dimocarpus confinis in Guangxi.Materials included 67 D.confinis individual plants,10 longan varieties,12 litchi varieties and 10 wild litchi plants in Guangxi and all the materials obtained clear fingerprints.A total of 151 major ISSR bands were observed,and 127 bands（84.11%） were polymorphic.All of the cultivars could be distinguished with the markers used.Genetic similarities among all D.confinis ranged from 0.61 to 0.97,and it indicated that D.confinis germplasm in Guangxi had abundant genetic diversity.There were obvious differences among D.confinis,longan and litchi,genetic similarities among all 99 taxa ranged from 0.53 to 0.98,litchi formed a single cluster and D.confinis and longan formed another cluster at Dice coefficient 0.53.The results showed that D.confinis was closer to longan in genetic material.

关 键 词：龙荔 遗传多样性 ISSR 广西 

分 类 号：S667.2[农业科学—果树学]