梨矮化砧木中矮1号GA20-氧化酶基因克隆与表达分析  

Isolation and expression analysis of gibberellin 20-oxidase gene from pear dwarfing Zhongai 1

作　　者：宣利利[1,2] 欧春青[1,2] 王斐[1,2] 王志刚[1,2] 程飞飞[1,2] 李振茹[3] 姜淑苓[1,2] 

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100 [2]农业部果树种质资源利用重点开放实验室,辽宁兴城125100 [3]北京市大兴区林业局,北京大兴102600

出　　处：《果树学报》2011年第5期883-887,共5页Journal of Fruit Science

基　　金：中央级公益性科研院所基本科研业务费专项;院区合作大兴区西洋梨产业升级关键技术研究与示范项目;农业科技成果转化资金项目(2010GB23260565)

摘　　要：中矮1号是中国农业科学院果树研究所选育的梨优良矮化砧木,为研究其矮化机理,利用RT-PCR结合RACE方法克隆了梨矮化砧木中矮1号的1个GA20-氧化酶基因,并对该基因及其氨基酸序列以及该基因在不同生长类型梨品种中不同时期新梢叶片中的表达进行了分析。结果表明,该基因全长1 179 bp,推导编码392个氨基酸,其编码的蛋白质具有GA20-氧化酶基因家族所具有的功能活性位点和特征保守单元,该基因与苹果GA20-氧化酶基因氨基酸序列一致性达91.58%,因此确定该基因为中矮1号GA20-氧化酶基因,将其命名为PcGA20ox1(Gen-Bank登录号为HQ833589)。PcGA20ox1基因在中矮1号、亲本锦香和对照早酥3个品种不同发育阶段新梢叶片中的表达强度均为中矮1号<锦香<早酥,与植株高矮表现一致,表明PcGA20ox1基因的表达强弱与植株高矮相关。To study the dwarfing mechanism of Zhongai 1 which was an excellent dwarfing rootstock bred by research institute of pomology,Chinese academy of agricultural sciences,gibberellin（GA） 20-oxidase gene was isolated from the young leaves of Zhongai 1 by reverse transcription polymerase chain reaction（RT-PCR）and rapid-amplification of cDNA end（RACE）,the gene,its putative amino acid sequences and its expression intensity at different growing stages of new leaves from various pears were analyzed,the results were as following： the full-length cDNA consists of 1 179 nucleotides and encodes a putative protein of 392 amino acid residues.The putative protein has all the conserved functional active sites and typical unit of the GA20-oxidase gene family.The putative protein sequence shared 91.58% identity with that of the protein found in apple.So we confirmed that the gene was the GA20-oxidase gene of Zhongai 1,and named it as PcGA20ox1（GenBank accession numbers was HQ833589）.The expression intensity of PcGA20ox1 in leaves of the three pear varieties had the same trend as Zhongai 1〈Jinxiang〈Zaosu in all tested growing periods,which were consistent with the plant height.This results indicated that the expression intensity of PcGA20ox1 was related with plant height as well.

关 键 词：梨 矮化砧木 赤霉素 GA20-氧化酶基因 基因表达 

分 类 号：S661.2[农业科学—果树学]