MC-002对大鼠局灶性脑缺血/再灌注治疗时间窗的研究  

Therapeutic time window of MC-002 on focal cerebral ischemia-reperfusion rats

作　　者：曾胜男 何广卫[2] 吴强[2] 袁晓艳[3] 吴玉林[4] 

机构地区：[1]湖南省邵东县中医院药剂科 [2]合肥医工医药有限公司暨安徽省药物再创新工程技术研究中心 [3]中南大学湘雅医院神经外科 [4]中国药科大学生理教研室

出　　处：《中国临床药理学与治疗学》2011年第8期867-873,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：十一.五重大专项课题号(2009ZX09103-123)

摘　　要：目的:研究受试药3-甲氧基-4-O-(2'-亚甲基-3'5'6'-三甲基吡嗪基)肉桂酸(MC-002)对大鼠局灶性脑缺血/再灌注损伤(CIRI)的治疗时间窗。方法:线栓法制作大鼠大脑中动脉闭塞(MCAO)2 h/再灌注22 h,并于复灌后0、0.5、1、2、4 h时治疗给药。观察MC-002对CIRI大鼠脑功能、脑梗死体积、脑含水量、组织形态学的影响以及MC-002各剂量组对大鼠脑组织内生化指标的影响。结果:MC-002在复灌后0、0.5、1、2 h给药能够显著性改善MCAO后大鼠的脑神经功能(P<0.05,P<0.01),降低大鼠的脑梗死率和含水量(P<0.05、P<0.01);增加CIRI大鼠脑组织超氧化物歧化酶(SOD)活力(P<0.01),降低丙二醛(MDA)及乳酸(LD)含量(P<0.05,P<0.01)。脑组织病理切片结果显示,和模型组比较,治疗组神经细胞形态和数目均得到显著改善。结论:MC-002 2 h内治疗给药对大鼠局灶性脑缺血/再灌注损伤有很好的保护作用。AIM： To investigate therapeutic time window of MC-002 on the focal cerebral ischemia-reperfusion rats. METHODS： The models with cerebral ischemicre-reperfusion were induced by intraluminal middle cerebral artery occlusion（MCAO） with a nylon monofilament suture,2 h occlusion followed by 22 h reperfusion.Therapeutic medicine was intravenously administered after reperfusion for 0,0.5,1,2,4 h.The effect of MC-002 at different doses on neurological scores,infarct sizes,water contents,pathological changes and the biochemical indexes of CIRI rats were examined.RESULTS：MC-002 administered at 0,0.5,1,2 h after reperfusion significantly improved the CIRI rats＇ neurological function and reduced the cerebral infarct size and edema;The content of MDA and LD in brain were markedly decreased（P0.05 and P0.01）,and the activity of SOD was significantly enhanced（P0.01）.The pathological observations showed that neuronal cells shape and number were obviously improved after treated with MC-002 compared with model groups.CONCLUSION： The results showed that the therapeutic time window of MC-002 extended for up to 2 h after CIRI.

关 键 词：MC-002 局灶性脑缺血/再灌注 治疗时间窗 

分 类 号：R965.2[医药卫生—药理学]