榄香烯对B细胞淋巴瘤Raji细胞株的体内外抑制作用  

In vivo and in vitro anti-tumor effects of elemene on B-cell lymphoma cell line Raji

作　　者：徐希[1] 庄彦[1] 章圣辉[2] 俞康[1] 

机构地区：[1]温州医学院附属第一医院血液科 [2]温州医学院附属第一医院医学科学研究所

出　　处：《中国临床药理学与治疗学》2011年第8期874-879,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省自然科学基金资助项目(302781)

摘　　要：目的:探讨榄香烯对B细胞淋巴瘤Raji细胞株的体内外作用。方法:不同浓度的榄香烯体外作用Raji细胞不同时间,CCK-8法检测增殖抑制作用,Annexin V/PI双染色法检测细胞凋亡,DAPI染色进行细胞核形态学分析,PI单染色法检测细胞周期改变;动物实验观察榄香烯体内抑瘤作用。结果:榄香烯对Raji细胞生长具有明显的抑制作用,呈量效和时效关系;榄香烯能诱导Raji细胞凋亡,呈量效关系;DAPI染色显示榄香烯作用Raji细胞具有凋亡细胞核的特征性核形态;细胞周期分析显示G0/G1期细胞相对增多、S期和G2/M期细胞均相对减少;榄香烯明显抑制Raji细胞在SCID小鼠体内增殖。结论:榄香烯对Raji细胞具有明显的增殖抑制作用,其机制可能是通过G0/G1期阻滞导致细胞凋亡发挥效用的。AIM： To investigate the inhibitory effects of elemene on B-cell lymphoma cell line Raji in vitro and in vivo. METHODS： Raji cells were incubated with indicated concentrations of elemene.The growth of Raji cells were observed by CCK-8 colorimetric assay,apoptosis rates were detected by flow cytometry using Annexin V-FITC/PI staining,analysis of nuclear morphology were observed with DAPI staining,cell cycles were analyzed by PI staining.And its in vivo anti-tumor effect on transplanted Raji cells in SCID mice was detected as well.RESULTS：Raji cells viability in presence of elemene decreased markedly in a dose and time dependent manner.The apoptosis could be induced by elemene and its level increased following the augmentation of the drug concentration.The characteristic apoptosis nuclei were observed in DAPI staining after elemene treatment.A concentration dependent increase of cells in G0/G1 phase,a decrease in S phase and G2/M phase were detected.Elemene had a great ability to inhibit the growth of Raji cells in SCID mice.CONCLUSION： Elemene can significantly inhibit the growth of Raji cells,its function is performed by inducing apoptosis and arresting cell cycles in G0/G1 phase.

关 键 词：榄香烯 RAJI细胞 细胞凋亡 动物实验 

分 类 号：R965.2[医药卫生—药理学]