胍丁胺对吗啡镇痛耐受大鼠脊髓和海马锌含量变化的影响  

Influence of agmatine on changes of zinc concentrations of spinal cord and hippocampus in morphine antinociceptivetolerance rats

作　　者：叶裕良 肖亮灿[2] 莫利求[2] 赵春梅[3] 郭瑞鲜[3] 陈培熹[3] 冯鉴强[3] 

机构地区：[1]东莞市横沥医院内科,广东东莞523460 [2]中山大学附属第一医院麻醉科,广东广州510080 [3]中山大学中山医学院生理学教研室,广东广州510080

出　　处：《解剖学研究》2011年第4期241-244,共4页Anatomy Research

基　　金：广东省科技计划项目(2010B080701035)

摘　　要：目的观察胍丁胺对吗啡耐受大鼠脊髓和海马组织中锌(Zn2+)含量变化的影响。方法采用SD成年雄性大鼠,建立慢性吗啡耐受大鼠模型,用热水甩尾法测定甩尾潜伏期观察痛反应的变化。动物随机分为4组:生理盐水(NS-NS)组、吗啡(NS-Mor)组、胍丁胺(Ag-NS)组、胍丁胺-吗啡(Ag-Mor)组。用原子吸收光谱法测定各组大鼠的脊髓和海马Zn2+含量。结果胍丁胺(10mg/kg)与吗啡合用具有显著的抗吗啡耐受作用,并阻断慢性吗啡耐受引起的脊髓和海马Zn2+含量的降低(P<0.001)。结论逆转吗啡耐受时脊髓和海马组织中Zn2+含量的减少,可能是胍丁胺抗吗啡耐受的机制之一。Objective To investigate influence of agmatine on changes of zinc levels of spinal cord and hippocampus inmorphine antinoceceptive tolerance rats. Methods In the adult Sprague-Dawley rats, chronic morphine tolerance rat model wasestablished. The analgesia effect was assessed by hot-water tail flick test. Animals were randomly divided into 4 groups ： saline-saline group, saline-morphine group, Agmatine-saline group and Agmatine-morphine group. The zinc concentrations in spinal cordand hippocampus were examined by atomic absorption spectrometry. Results Agmatine （10 mg / kg） coadministrated with morphinesignificantly antagonized morphine tolerance along with blocking chronic morphine-induced reduction of zinc levels in the spinal cordand hippocampus （P0.001）. Conclusion One of the mechanisms underlying the blockage of morphine tolerance by agmatine is toreverse the decrease in zinc levels induced by morphine tolerance.

关 键 词：吗啡 胍丁胺 锌 脊髓 海马 

分 类 号：R96[医药卫生—药理学]