急性心肌缺血WHBE兔心脏血流动力学指标的观测与比较  

Observation and Comparasion on Cardiac Hemodynamics in WHBE Rabbit with Acute Myocardial Ischemia

作　　者：鲁珽[1] 潘永明[1] 陈民利[1] 陈亮[1] 徐剑钦[1] 何欢[1] 王德军[1] 应华忠[1] 

机构地区：[1]浙江中医药大学动物实验研究中心／比较医学研究中心,杭州310053

出　　处：《实验动物与比较医学》2011年第4期264-268,共5页Laboratory Animal and Comparative Medicine

基　　金：1.浙江省中医药科学研究基金计划（2010ZB032）,2.浙江省公共科技基础平台项目（Dwpt-bz-12）,3.受“浙江省卫生高层次创新人才培养工程项目”资助

摘　　要：目的 观察WHBE兔急性心肌缺血模型心脏血流动力学的变化,并与日本大耳白兔比较,探讨其血流动力学的差异.方法 雄性WHBE兔和日本大耳白兔各20只,随机分成伪手术组和模型组,每组10只,伪手术组只开胸不结扎,模型组采用冠脉结扎复制心肌缺血模型,观察实验兔血流动力学的变化.结果 与日本大耳白兔比,麻醉下WHBE兔LVSP、LVDP、Lo、-dp/dt（max）和RPP均显著高于日本大耳白兔（P〈0.05,P〈0.01）,但HR、SAP、DAP、MAP、＋dp/dt（max）、LVEDP、Tau差异不显著（P〉0.05）.与伪手术组比,冠脉结扎后WHBE兔和日本大耳白兔模型组的SAP、DAP、MAP、LVSP、LVSP、＋dp/dt（max）、LVDP、Lo、RPP的变化率均显著降低（P〈0.05,P〈0.01）,LVEDP、Tau的变化率均显著升高（P〈0.05,P〈0.01）,且HR亦有降低趋势,但差异不显著（P〉0.05）;与日本大耳白兔组比,冠脉结扎60min后WHBE兔的血流动力学参数均略低于日本大耳白兔（P〉0.05）.结论 WHBE兔对冠脉结扎后血流动力学改变明显,或可用于杭心肌缺血药物的评价和机制研究.Objective To observe the change of cardiac hemodynamics in WHBE rabbit acute myocardial ischemia model induced by ligation of coronary artery, and compare it with Japanese white rabbit to investigate the difference of the cardiac hemodynamics. Methods Male WHBE rabbits and Japanese white rabbits were randomly divided into sham operation group and model group （ =10 in each）. The rabbits sham operation group were only opened chest but not ligated, and the myocardial infarction model was induced by ligation of coronary artery. The cardiac hemodynamics was observed. Results LVSP, LVDP, Lo, -dp/dtmax and RPP in normal anesthetized WHBE rabbits were significantly higher than those of Japanese white rabbits （P〈0.05, P〈0.01）, but HR, DAP, MAP, ＋dp/dtmax, LVEDP and Tau has no significant difference （P〉0.05）.Compared with sham operation group, the change rates of SAP, DAP, MAP, LVSP, ＋dp/dt -dp/dt LVDP, Lo, RPP in WHBE rabbits and Japanese white rabbits model groups after ligation of coronary artery were significantly lower than those of sham opera- tion groups （P〈0.05, P〈0.01）, while the change rates of LVEDP and Tan significantly increase （P〈0.05, P〈0.01）, and HR was trend to decrease （P〉0.05）. The cardiac hemodynamics parameters in WHBE rabbits after ligated for 60 min were slightly lower than those of Japanese white rabbits （P〉0.05）. Conclusion WHBE rabbit has a significant hemodynamics changes after ligated coronary artery, this model can be used in evaluation of anti-myocardial ischemia drug and mechanism research.

关 键 词：WHBE兔 心脏血流动力学 心肌缺血 日本大耳白兔 动物模型 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]