Age-dependent expression of forkhead box O proteins in the duodenum of rats  

作　　者：Pan HUANG Zhen-qi ZHOU Rui-hua HUANG Bo ZHOU Quan-wei WEI Fang-xiong SHI 

机构地区：[1]College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2011年第9期730-735,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (No. 30771553);the Basic Research Foundation for Science and Technology of Nanjing Agricultural University,China

摘　　要：The O subfamily of forkhead box(FoxO) proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B(IGF-1/PI3K/PKB) signal pathway.The objective of the present study was to examine the expressions of three members of FoxO proteins,FoxO1,FoxO3a,and FoxO4 in the duodenum of Sprague-Dawley rats at different ages.The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner.At Day 21,there were no detectable localization and expression of FoxO4 in the duodenum,while,at Months 2 and 6,localization and expression of FoxO4 were distinct.In addition,FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats,but was not detectable in the same area in 6-month-old rats.Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2-and 6-month marks,it was still not detectable at Day 21.Besides,FoxO1 was not detectable in all parts and stages.Taken together,our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.The O subfamily of forkhead box（FoxO） proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B（IGF-1/PI3K/PKB） signal pathway.The objective of the present study was to examine the expressions of three members of FoxO proteins,FoxO1,FoxO3a,and FoxO4 in the duodenum of Sprague-Dawley rats at different ages.The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner.At Day 21,there were no detectable localization and expression of FoxO4 in the duodenum,while,at Months 2 and 6,localization and expression of FoxO4 were distinct.In addition,FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats,but was not detectable in the same area in 6-month-old rats.Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2-and 6-month marks,it was still not detectable at Day 21.Besides,FoxO1 was not detectable in all parts and stages.Taken together,our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.

关 键 词：O subfamily of forkhead box（FoxO） AGE-DEPENDENCE DUODENUM Rat 

分 类 号：Q593[生物学—生物化学]