低温对百合鳞茎内源激素含量变化的影响  

Effects of Low Temperature on Change of Endogenous Hormone Contents in Lily Bulb

作　　者：李翊华[1,2] 安丽萍[3] 李彬[1] 谢忠奎[3] 

机构地区：[1]甘肃农业大学农学系,甘肃兰州730070 [2]河西学院,甘肃张掖734000 [3]中国科学院寒区旱区环境与工程研究所皋兰生态与农业综合试验站,甘肃兰州730000

出　　处：《中国沙漠》2011年第5期1208-1214,共7页Journal of Desert Research

基　　金：中国科技部农业成果转化项目(2007GB24910484);中国科学院重要方向性项目(KSCX2-YW-N-44-07);科技支甘项目(KJZG-2008-3)资助

摘　　要：以东方百合Siberia和Tiber为试验材料,进行恒温和变温的低温处理,采用高效液相色谱法测定在不同冷藏解除休眠时期赤霉素(GA3)、吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(ZT)4种内源激素含量。结果表明,恒温处理Tiber先解除休眠,同样处理同期种植的百合鳞茎,Tiber比Siberia一般早出苗7 d左右;变温处理比恒温处理ABA含量降低,说明变温处理对百合鳞茎解除休眠有促进作用,可以缩短解除休眠需要的时间,一般变温处理的比恒温处理的早8 d出苗,且变温处理有利于百合生长量的增加,Tiber茎粗和株高分别增加了23.1%、10.1%,同时Siberia分别增加了5.9%和16.6%。变温处理GA3含量Tiber高于恒温处理,Siberia低于恒温处理,品种之间对温度处理的响应有差异。百合鳞茎休眠的解除与IAA/ABA、ZT/ABA、GA3/ABA比值有关,变温Siberia和Ti-ber的3种比值都在60 d后逐渐升高,说明冷藏60 d可作为鳞茎解除休眠的临界点,为进一步研究百合鳞茎的休眠机理提供了重要线索。Contents of four endogenous hormones,namely Zeatin（ZT）,Gibberellin（GA3）,Indoleacetic Acid（IAA）,Abscisic Acid（ABA）,in Oriental Lily of Siberia and Tiber under constant low temperature and variable low temperature treatments were determined using High Performance Liquid Chromatography.Results show that Tiber relieves dormancy first under constant low temperature treatment,and it emerges 7 d earlier than Siberia;ABA content under variable low temperature treatment is less than under constant low temperature treatment,and it shows that variable low temperature treatment can promote the relieving of dormancy and shorten the time required for relieving dormancy of the lily bulbs.Lily emerges 8 d earlier under variable low temperature treatment than under constant low temperature treatment.Lily grows better under variable low temperature treatment than under constant low temperature treatment,and increments of stem diameter and plant height of Tiber increases are 23.1% and 10.1%,respectively,while that of Siberia are 5.9% and 16.6%,respectively.GA3 content in Tiber under variable low temperature treatment is higher than under constant low temperature treatment,while GA3 content in Siberia under variable low temperature treatment is lower than under constant low temperature treatment.Dormancy release of Lily Bulb is related to ratios of IAA/ABA,ZT/ABA,GA3/ABA,and the three ratios of Siberia and Tiber increase gradually under variable low temperature treatment of 60 d,indicating 60 d of low temperature can be used as the critical point for dormancy relieving of Lily Bulb.The paper provides a clue for further study of the dormancy release mechanism of Lily Bulb.

关 键 词：低温 鳞片 休眠 内源激素 

分 类 号：Q945[生物学—植物学]