T3调控氟他胺诱导大鼠隐睾生殖母细胞发育障碍及NCAM表达  

The Regulation of T3 on Maldeveloped Gonocyte(Go) and Expression of NCAM in Flutamide-induced Cryptorchidism Rat

作　　者：马超[1,2] 何大维[1] 姜勇超[1,3] 赵丹[1] 何文飞[1] 魏光辉[1] 

机构地区：[1]重庆医科大学附属儿童医院泌尿外科儿童发育疾病研究省部共建教育部重点实验室,重庆400014 [2]成都市妇女儿童中心医院小儿外科,成都610031 [3]襄阳市中心医院儿科,襄阳441021

出　　处：《四川大学学报（医学版）》2011年第5期638-641,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号81170556);重庆市自然科学基金(CSTC 2010BB5112)资助

摘　　要：目的观察三碘甲腺原氨酸(T3)对氟他胺(Flu)诱导的SD大鼠隐睾生殖母细胞(Go)发育及神经细胞黏附分子(NCAM)表达的调控作用。方法 15μg/(kg.d)T3皮下注射Flu诱导出生后第1 d(PD1)仔鼠、PD20隐睾仔鼠,观察干预后隐睾发生率、组织学改变,免疫组化及逆转录聚合酶链反应(RT-PCR)检测NCAM表达。结果 Flu诱导仔鼠PD1给予T3干预,成年后隐睾发生率为25%(8/32),较未干预组的隐睾发生率40.9%(9/22)降低,且隐睾曲细精管内无残留生殖母细胞(Go),NCAM阴性表达。Flu诱导的PD13仔鼠睾丸组织、PD20仔鼠隐睾组织NCAMmRNA表达高于正常组(P<0.01,P<0.05),T3干预后,NCAMmRNA表达均低于非T3干预组(P<0.01),并与正常组无明显差异(P>0.05)。结论 T3可以降低Flu诱导暴露后隐睾发生率,下调NCAM表达,降低或消除隐睾曲细精管Go残存。Objective To investigate the regulation of T3 on the maldeveloped gonocytes（Go） and the expression of NCAM in Flutamide-induced cryptorchidism SD rat.Methods Postnatal day（PD） 1 and PD20 cryptorchidism SD rat induced by Flu were treated with 15 μg/（kg·d） T3 by subcutaneous injections.Incidence of the cryptorchidism,histology were evaluated.And the expression of NCAM was detected by immunohistochemisty and RT-PCR.Results The incidence of cryptorchidism in the group of Flu-induced offsprings treated by 15 μg/（kg·d） T3 from PD1 was 25%（8/32）,which was lower than that of Flu group 40.9%（9/22）.No Go and NCAM expression were observed in the seminiferous tubules.NCAM mRNA expression in Flu-induced PD13,PD20 testes was up-regulated when compared with control（P0.01,P0.05）,while after treated with 15 μg/（kg·d） T3 the NCAM expression was significantly down-regulated compared to that of non T3 treated group（P0.01）,and was not significantly different from that of control（P0.05）.Conclusion The incidence of Flu-induced cryptorchidism can be decreased by T3 treatment after birth.T3 may contribute to the down-regulation of the NCAM expression,and reduction or elimination of the remainded gonocytes in crytorcidism seminiferous tubules.

关 键 词：氟他胺 隐睾 三碘甲腺原氨酸 生殖母细胞 神经细胞黏附分子 

分 类 号：R726.9[医药卫生—儿科]