柴芩承气汤对急性胰腺炎小鼠血清胆囊收缩素-8水平及胰腺腺泡钙超载的影响  

Effect of Chai Qin Cheng Qi Decoction on Serum CCK-8 and Calcium Overload of Pancreatic Acinar in Mice with Acute Pancreatitis

作　　者：郭佳[1] 张海燕[1] 金涛[1] 赵龙[1] 文礼[1] 黄蕾[1] 薛平[1] 程征宇[1] 杨晓楠[1] 夏庆[1] 

机构地区：[1]四川大学华西医院中西医结合科,成都610041

出　　处：《四川大学学报（医学版）》2011年第5期704-706,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号81072910);四川省科技支撑计划项目(No.2009SZ0201;No.2010SZ0068)资助

摘　　要：目的探讨柴芩承气汤(CQCQD)对急性胰腺炎(AP)小鼠血清胆囊收缩素(CCK)-8及胰腺腺泡细胞内钙离子浓度的影响。方法将24只小鼠随机分成对照组、AP组、CQCQD组及siRNA组(n=6)。后3组采用腹腔内注射8%左旋精氨酸溶液(4 g/kg)制作AP小鼠模型。对照组注射相同剂量的生理盐水。造模成功后,CQCQD组予以管喂0.4 mL/100 g体质量的CQCQD,1次/2 h,共3次;siRNA组予以腹腔注射0.88 mg/kgCCK-siRNA,AP组腹腔注射相同体积的生理盐水。观察干预后6 h各组血清CCK-8及胰腺腺泡细胞钙离子浓度及胰腺病理改变。结果AP组血清CCK-8浓度〔(3764.3±369.2)ng/mL〕、胰腺腺泡细胞钙离子荧光强度(34.8±27.1)及胰腺组织病理评分(6.2±1.1)与对照组〔(1253.5±39.5)ng/mL,5.2±2.3,2.8±0.4〕、CQCQD组〔(1230.5±46.1)ng/mL,9.6±1.6,3.8±0.8〕及siRNA组〔(1702.3±598.3)ng/mL,7.6±2.0,4.1±0.5〕相比较差异有统计学意义(P<0.05)。血清CCK-8与胰腺腺泡细胞内钙离子浓度(r=0.793,P=0.021)及胰腺组织病理评分(r=0.847,P=0.000)呈正相关。结论精氨酸诱导AP小鼠模型与血清CCK-8浓度升高、诱发胰腺腺泡细胞钙超载有关,CQCQD可以降低AP小鼠血清CCK-8水平,缓解胰腺腺泡细胞钙超载,减轻胰腺病理改变。Objective To investigate the effect of Chai Qin Cheng Qi decoction（CQCQD） on serum cholecystokinin-8（CCK-8） and calcium overload of pancreatic acinar cells in acute pancreatitis（AP） mice.Methods Twenty four mice were randomly divided into control group,AP group,CQCQD group and siRNA group,each comprising 6 mice.AP mouse model was induced by intraperitoneal injection of 8% L-arginine in a dose of 4 g/kg.The AP mice in the CQCQD group were fed with 0.4 mL/100 g of Chai Qin Cheng Qi solution once every two hours.The AP mice in the siRNA group were injected intraperitoneally with CCK-siRNA in a dose of 0.88 mg/kg.The changes of serum CCK-8 and calcium concentrations in the pancreatic acinar cells and pancreatic pathology were observed 6 hours after the interventions.Results The serum CCK8（（3764.3±369.2） ng/mL）,calcium fluorescence intensity（34.8±27.1） of pancreatic acinar cells and pancreas pathology scores（6.2±1.1） of the AP mice were significantly greater（P0.05） than those in the control group（（1253.5±39.5） ng/mL,5.2±2.3,2.8±0.4）,CQCQD group（（1230.5±46.1） ng/mL,9.6±1.6,3.8±0.8,4.1±0.5） and siRNA group（（1702.3±598.3） ng/mL,7.6±2.0）.Serum CCK-8 was positively correlated with intracellular calcium concentrations（r=0.793,P=0.021） in pancreatic acinar cells and pancreas pathology scores（r=0.847,P=0.000）.Conclusion Acute pancreatitis in mice induced by L-arginine is associated with calcium overload in pancreatic acinar cells induced by increased serum CCK-8.CQCQD can reduce serum levels of CCK-8,alleviate calcium overload in pancreatic acinar cells,and reduce pancreas pathological changes in AP mice.

关 键 词：急性胰腺炎 柴芩承气汤 胆囊收缩素-8 胆囊收缩素RNA干扰 钙超载 

分 类 号：R285.5[医药卫生—中药学]