VEGF-A、VEGF-C及PLGF基因在奶牛乳腺发育各时期的表达变化  

Expression Changes of VEGF-A,VEGF-C and PLGF Gene in Different Developmental Periods of Dairy Cow Mammol/lary Gland

作　　者：张岚[1] 李庆章[1] 边艳杰[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《中国畜牧兽医》2011年第9期52-56,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点基础研究发展(973)计划(2011CB100800);东北农业大学创新团队项目(CXT005-1-1/CXT005-1-2)

摘　　要：试验旨在研究奶牛VEGF-A、VEGF-C及PLGF基因在乳腺发育各个时期的mRNA表达情况,进而为下一步研究奶牛乳腺发育功能基因奠定基础。采用Real-time PCR SYBR GreenⅠ染料法,所得结果与Affymetrix公司的基因芯片差异筛选结果作比较分析。结果表明,3个基因在乳腺发育11个时期均有表达。VEGF-A基因在妊娠后期及泌乳期表达量较高且与其它时期差异显著(P<0.05);VEGF-C基因在泌乳期表达量较高;PLGF基因的表达量从青春期开始到妊娠期呈递增趋势,到妊娠期4个月达到最高表达量,其后表达量逐渐递减。从以上结果可以得出,VEGF-A及VEGF-C基因可能在奶牛泌乳中发挥作用,而PLGF基因对奶牛乳腺发育起到重要作用,因此通过PCR扩增出PLGF基因的全部CDS序列,为下一步研究PLGF基因功能奠定基础。In order to study the mRNA expression changes of VEGF-A,VEGF-C,and PLGF gene in different developmental periods of dairy cow mammol/lary gland,then lay the foundation for further research on the gene function of the cow mammol/lary gland.The SYBR Green Ⅰ dye Real-time PCR method was used,and the results were compared,with gene chip differential screening results of Affymetrix company.The results showed that three genes were expressed in 11 periods of mammol/lary gland.VEGF-A gene expression during late pregnancy and lactation was higher and significantly different from other periods（P0.05）;VEGF-C gene expression was higher during lactation;PLGF gene expression from the onset of virgin to pregnancy increases gradually to 4 months pregnancy the highest expression level,followed by gradually decreasing the expression.The results could be drawn from the above,VEGF-A and VEGF-C gene may play a role in the lactating cows,and PLGF gene might be play an important role to cow mammol/lary gland development,so get the PLGF CDS sequence by PCR amplified method,lay the test foundation for further research on the gene function of PLGF gene.

关 键 词：VEGF基因 荧光定量PCR 乳腺上皮细胞 差异表达 

分 类 号：Q786[生物学—分子生物学]