牛奶中邻苯二甲酸酯类环境激素测定方法研究  

Determination of Phthalate Acid Esters Plasticizers in Milk

作　　者：杨柳[1] 杨凯[1] 路平[1] 于同泉[1] 王建立[1] 

机构地区：[1]北京农学院农业应用新技术北京市重点实验室,北京102206

出　　处：《中国畜牧兽医》2011年第9期74-79,共6页China Animal Husbandry & Veterinary Medicine

基　　金：北京农学院青年基金项目"环境激素-邻苯二甲酸酯类在乳牛饲料;乳牛脏器和牛乳中的含量检测"(06-QN-SW-01)

摘　　要：采用气相色谱/质谱法测定牛奶中涉及美国国家环保署(EPA)优先控制污染的6种邻苯二甲酸酯类环境激素,分别为邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)、邻苯二甲酸正二丁酯(DnBP)、邻苯二甲酸丁基苄基酯(BBP)、邻苯二甲酸二(2-乙基)己酯(DEHP)和邻苯二甲酸二正辛酯(DnOP)。室温振荡提取方式优于超声波提取,用正己烷作提取溶剂,室温振荡提取1.5h,就可将牛奶中的邻苯二甲酸酯类化合物提取完全。精密度检验和添加回收试验结果表明,PAEs各化合物的回收率在85.00%～102.14%之间,标准偏差(RSD)在1.88%～5.50%之间,说明本方法准确可靠。应用本方法检测市售牛奶中PAEs,DEP和DnBP检出频率较多,BBP检出量最大,塑料包装的袋奶主要检出BBP、DEHP和DnOP,且比其它包装的牛奶含量都高,说明包装材料中的PAEs对牛奶有污染作用,需要引起足够重视。The contents of phthalate acid esters（PAEs） BBP,DBP,DEHP,DNOP,DINP and DIDP in milk were investigated by GC/MS.According to the U.S.environmental protection agency（EPA）,the extraction method of room temperature oscillation was better than ultrasonic.The milk samples pre-treatment were extracted with n-Hexane using constant temperature shocker at room temperature 1.5 h.The recovery rate of each PAEs were 85.00% to 102.14%,relative standard deviation（RSD） values were 1.88% to 5.50%.The results indicated that the method was effectively to detect the PAEs in milk,and this method was convenient,accurate and relable.The high detectable rate of AEs,DEP and DnBP in marker milk,the largest detected quantity of EPA was BBP.EHP and DnOP were detected in plastic bags milk,and the amount of them were higher than other packaging＇s.It was indicated that the packaging materials could contaminated milk,and need to cause enough attention.

关 键 词：牛奶 邻苯二甲酸酯类 环境激素 

分 类 号：S851.433[农业科学—预防兽医学]